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Napsin A antibody [NAPSA/1238]

Anti-Napsin A antibody [NAPSA/1238] used in IHC (Paraffin sections) (IHC-P). GTX34887
Anti-Napsin A antibody [NAPSA/1238] used in Western Blot (WB). GTX34887

Cat No. GTX34887

Host Mouse
Clonality Monoclonal
Clone Name NAPSA/1238
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2ug/ml for 60 minutes at RT
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Specificity/Sensitivity

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)

Purification

Ab purified from rabbit anti-serum by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Napsin A Aspartic Peptidase,Kap,Kdap,Nap1,Napa,Snapa,Napsa

Background

The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Napsin A antibody [NAPSA/1238] used in IHC (Paraffin sections) (IHC-P). GTX34887

GTX34887 IHC-P Image

Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1238).

Anti-Napsin A antibody [NAPSA/1238] used in Western Blot (WB). GTX34887

GTX34887 WB Image

Western Blot of K562, HEK293 andA549 Cell Lysates Using Napsin-A Monoclonal Antibody (NAPSA/1238)

Package List Price ($)
$ 329