*Optimal dilutions/concentrations should be determined by the researcher.
|1-2ug/ml for 60 minutes at RT
|1-2ug/ml for 30 minutes at RT
Boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)
Ab purified from rabbit anti-serum by Protein A
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Napsin A Aspartic Peptidase,Kap,Kdap,Nap1,Napa,Snapa,Napsa
The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [provided by RefSeq, Jul 2008]
GTX34887 IHC-P Image
Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1238).
GTX34887 WB Image
Western Blot of K562, HEK293 andA549 Cell Lysates Using Napsin-A Monoclonal Antibody (NAPSA/1238)