APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml for 60 minutes at RT |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
KAP , Kdap , NAP1 , NAPA , NAPSA , SNAPA , napsin A aspartic peptidase , Napsin A
Cellular Localization
Secreted
Background
This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
![Anti-Napsin A antibody [NAPSA/1239] used in IHC (Paraffin sections) (IHC-P). GTX34888](/templates/default/js/lightbox/images/loading.gif "Anti-Napsin A antibody [NAPSA/1239] used in IHC (Paraffin sections) (IHC-P). GTX34888")
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GTX34888 IHC-P Image
IHC-P analysis of human lung adenocarcinoma tissue using GTX34888 Napsin A antibody [NAPSA/1239].
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GTX34888 WB Image
WB analysis of K562 and HEK293 cell lysates using GTX34888 Napsin A antibody [NAPSA/1239].
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REFERENCE
There are currently no references for Napsin A antibody [NAPSA/1239] (GTX34888). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Napsin A antibody [NAPSA/1239] (GTX34888). Be the first to share your experience with this product.
![Anti-Napsin A antibody [NAPSA/1239] used in IHC (Paraffin sections) (IHC-P). GTX34888](/upload/website/prouct_img/small/GTX34888/GTX34888_20200115_IHC-P_670_w_23060801_242.webp "Anti-Napsin A antibody [NAPSA/1239] used in IHC (Paraffin sections) (IHC-P). GTX34888")
![Anti-Napsin A antibody [NAPSA/1239] used in Western Blot (WB). GTX34888](/upload/website/prouct_img/small/GTX34888/GTX34888_20200115_WB_1489_w_23060801_818.webp "Anti-Napsin A antibody [NAPSA/1239] used in Western Blot (WB). GTX34888")
![Anti-Napsin A antibody [NAPSA/1239] used in IHC (Paraffin sections) (IHC-P). GTX34888](/upload/website/prouct_img/normal/GTX34888/GTX34888_20200115_IHC-P_670_w_23060801_242.webp "Anti-Napsin A antibody [NAPSA/1239] used in IHC (Paraffin sections) (IHC-P). GTX34888")
![Anti-Napsin A antibody [NAPSA/1239] used in Western Blot (WB). GTX34888](/upload/website/prouct_img/normal/GTX34888/GTX34888_20200115_WB_1489_w_23060801_818.webp "Anti-Napsin A antibody [NAPSA/1239] used in Western Blot (WB). GTX34888")