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Nav1.6 antibody

Anti-Nav1.6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54842
Anti-Nav1.6 antibody used in Western Blot (WB). GTX54842
Anti-Nav1.6 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54842

Cat. No. GTX54842

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all Nav1.6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

225 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide CIANHTGVDIHRNGDFQKNG, corresponding to amino acid residues 1042-1061 (Intracellular loop between domains II and III) of rat NaV1.6 (Accession  : O88420).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887892

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sodium voltage-gated channel alpha subunit 8

Cellular Localization

Cell membrane

Background

tetrodotoxin-sensitive sodium channel alpha subunit [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Nav1.6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54842

GTX54842 ICC/IF Image

ICC/IF analysis of PFA-fixed rat DRG primary cells using GTX54842 Nav1.6 antibody.
Panel A : Primary antibody
Panel B : DNA dye Hoechst 33342
Panel C : Merged images of panels A and B
Dilution : 1:200

Anti-Nav1.6 antibody used in Western Blot (WB). GTX54842

GTX54842 WB Image

WB analysis of rat brain membrane lysates using GTX54842 Nav1.6 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-Nav1.6 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54842

GTX54842 IHC-Fr Image

IHC-Fr analysis of mouse hippocampus tissue using GTX54842 Nav1.6 antibody. DAPI is used as the counterstain.
Panel A : NaV1.6 (green) is robustly expressed in the CA1 pyramidal layer (white arrows).
Panel B : Staining with mouse anti-parvalbumin (red), a marker of interneurons.
Panel C : Merged image of panels A and B  reveals that NaV1.6 appears in some interneurons (arrow) but is not restricted to interneurons.

REFERENCE

Application Reference

S?nchez-Aguilera A et al. Neuroscience 2020; 442 : 151-167 Development of Action Potential Waveform in Hippocampal CA1 Pyramidal Neurons.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639