Nck beta antibody

Cat No. GTX33346

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($339)

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Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
IHC-P	1:50 - 1:200

Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human NCK2 (NP_003572.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NCK adaptor protein 2 , GRB4 , NCKbeta

Cellular Localization

Cytoplasm,Endoplasmic reticulum

Background

This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Gene ID: 8440 NCK2
UniProt: O43639 NCK2

Research Area

DATA IMAGES

GTX33346 WB Image

WB analysis of various sample lysates using GTX33346 Nck beta antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

GTX33346 IHC-P Image

IHC-P analysis of human prostate tissue using GTX33346 Nck beta antibody.
Dilution : 1:100

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 339

Qty