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Nectin 1 antibody

Anti-Nectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130824
Anti-Nectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130824
Anti-Nectin 1 antibody used in Western Blot (WB). GTX130824
Anti-Nectin 1 antibody used in Western Blot (WB). GTX130824

Cat. No. GTX130824

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

RT-4, Mouse brain, rat brain

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human PVRL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886355

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nectin cell adhesion molecule 1 , CD111 , CLPED1 , ED4 , HIgR , HV1S , HVEC , OFC7 , PRR , PRR1 , PVRL1 , PVRR , PVRR1 , SK-12 , nectin-1

Cellular Localization

Isoform Alpha: Cell membrane; Single-pass type I membrane protein , Isoform Delta: Cell membrane; Single-pass type I membrane protein , Isoform Gamma: Secreted

Background

This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Nectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130824

GTX130824 ICC/IF Image

Nectin 1 antibody detects Nectin 1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nectin 1 protein stained by Nectin 1 antibody (GTX130824) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Nectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130824

GTX130824 ICC/IF Image

Nectin 1 antibody detects Nectin 1 protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nectin 1 stained by Nectin 1 antibody (GTX130824) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Nectin 1 antibody used in Western Blot (WB). GTX130824

GTX130824 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin 1 antibody (GTX130824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 12686612

Anti-Nectin 1 antibody used in Western Blot (WB). GTX130824

GTX130824 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin 1 antibody (GTX130824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169