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Nectin 1 antibody

Anti-Nectin 1 antibody used in Western Blot (WB). GTX130824
Anti-Nectin 1 antibody used in Western Blot (WB). GTX130824
Anti-Nectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130824

Cat No. GTX130824

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Nectin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nectin cell adhesion molecule 1,CD111,CLPED1,ED4,HIgR,HV1S,HVEC,OFC7,PRR,PRR1,PVRL1,PVRR,PVRR1,SK-12,nectin-1

Cellular Localization

Isoform Alpha: Cell membrane; Single-pass type I membrane protein , Isoform Delta: Cell membrane; Single-pass type I membrane protein , Isoform Gamma: Secreted

Background

This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Nectin 1 antibody used in Western Blot (WB). GTX130824

GTX130824 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin 1 antibody (GTX130824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nectin 1 antibody used in Western Blot (WB). GTX130824

GTX130824 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PVRL1 antibody (GTX130824) diluted at 1:500.

Anti-Nectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130824

GTX130824 ICC/IF Image

Nectin 1 antibody detects Nectin 1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nectin 1 protein stained by Nectin 1 antibody (GTX130824) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf