APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody will recognize all known isoforms of Nectin-1, including the secreted form (also known as isoform gamma).
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.8 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide (C)GKPPSVVSWETRLK, corresponding to amino acid residues 177-190 (Extracellular, N-terminus) of human Nectin-1 (Accession : Q15223).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nectin cell adhesion molecule 1 , CD111 , CLPED1 , ED4 , HIgR , HV1S , HVEC , OFC7 , PRR , PRR1 , PVRL1 , PVRR , PVRR1 , SK-12 , nectin-1
Background
This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
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GTX54843 LCI Image
Live cell imaging analysis of live intact PC12 cells using GTX54843 Nectin 1 antibody.
Panel A : Primary antibody (Red)
Panel B : Live view of the cell
Panel C : Merged images of Panel A and B
Dilution : 1:100
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GTX54843 FACS Image
FACS analysis of THP-1 cells using GTX54843 Nectin 1 antibody.
Black : Unstained cell
Green : Cell staining with primary antibody
Dilution : 1:15
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GTX54843 WB Image
WB analysis of Jurkat (lanes 1 and 3) and MEG-01 (lanes 2 and 4) cell lysates using GTX54843 Nectin 1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200
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GTX54843 WB Image
WB analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates using GTX54843 Nectin 1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200
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GTX54843 IHC (Free Floating) Image
IHC-FoFr (Free Floating) analysis of rat cerebellum tissue using GTX54843 Nectin 1 antibody.
Panel A: Nectin-1 staining (red) is apparent in Purkinje neurons and their dendritic tree (arrow).
Panel B : Cell nuclei in the same section are visualized with DAPI (blue).
Panel C : Merge of the two images.
Dilution : 1:100
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REFERENCE
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REVIEW
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_396_w_23060900_120.webp "Anti-Nectin 1 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX54843")
_396_w_23060900_120.webp "Anti-Nectin 1 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX54843")