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Nectin 3 antibody

Anti-Nectin 3 antibody used in Western Blot (WB). GTX103654
Anti-Nectin 3 antibody used in Western Blot (WB). GTX103654

Cat No. GTX103654

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

K562 , THP-1 , mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nectin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nectin cell adhesion molecule 3 , CD113 , CDW113 , NECTIN-3 , PPR3 , PRR3 , PVRL3 , PVRR3

Cellular Localization

Cell membrane; Single-pass membrane protein

Background

Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-Nectin 3 antibody used in Western Blot (WB). GTX103654

GTX103654 WB Image

Sample (30 ug of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX103654 diluted at 1:10000

Anti-Nectin 3 antibody used in Western Blot (WB). GTX103654

GTX103654 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nectin 3 antibody (GTX103654) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169