*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
180 kDa. The observed M.W. is based on the following publication. PMID: 16603261
293T , mouse brain , rat brain
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.33mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human Nek1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DKFZp686D06121 antibody , DKFZp686K12169 antibody , KIAA1901 antibody , MGC138800 antibody , NIMA (never in mitosis gene a)-related kinase 1 antibody , NIMA related kinase 1 antibody , NY-REN-55 antibody , NY\ REN55 antibody , Nek1 antibody , Nek1 antibody , SRPS2 antibody , SRPS2A antibody , never in mitosis A-related kinase 1 antibody , nimA-related protein kinase 1 antibody , protein-serine/threonine kinase antibody , renal carcinoma antigen NY-REN-55 antibody , serine/threonine-protein kinase Nek1 antibody
The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX130828 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 16603261
GTX130828 ICC/IF Image
Nek1 antibody detects Nek1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nek1 protein stained by Nek1 antibody (GTX130828) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX130828 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.