GeneTex
United States (US)

Nek1 antibody

GTX130828_42375_20160331_WB_18121410_175.jpg
GTX130828_42375_2016518_IFA_18121410_612.jpg
GTX130828_42375_20160414_WB_M_R_18121410_667.jpg
GTX130828_42375_20190201_WB_M_tissue_19022001_322.jpg

Cat No. GTX130828

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Observed MW

180 kDa. The observed M.W. is based on the following publication. PMID: 16603261

Positive Control

293T , mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nek1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nima Related Kinase 1,Als24,Ny-Ren-55,Srps2,Srps2A,Srtd6,Nek1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX130828_42375_20160331_WB_18121410_175.jpg

GTX130828 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 16603261

GTX130828_42375_2016518_IFA_18121410_612.jpg

GTX130828 ICC/IF Image

Nek1 antibody detects Nek1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nek1 protein stained by Nek1 antibody (GTX130828) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX130828_42375_20160414_WB_M_R_18121410_667.jpg

GTX130828 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.

GTX130828_42375_20190201_WB_M_tissue_19022001_322.jpg

GTX130828 WB Image

Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169