Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Neogenin antibody [N1N2], N-term (GTX109043) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.