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Nephrin antibody

Anti-Nephrin antibody used in IHC (Paraffin sections) (IHC-P). GTX31936
Anti-Nephrin antibody used in Western Blot (WB). GTX31936
Anti-Nephrin antibody used in IHC (Paraffin sections) (IHC-P). GTX31936

Cat. No. GTX31936

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Nephrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

135 kDa. ( Note )

Positive Control

Human Kidney Tissue Lysate(GTX29995) , Human Kidney Tissue Slide

Product Note

Nephrin antibody is human specific.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Nephrin antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Nephrin.The immunogen is located within amino acids 1100 - 1150 of Nephrin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NPHS1 adhesion molecule, nephrin , CNF , NPHN , nephrin

Cellular Localization

Cell membrane

Background

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-Nephrin antibody used in IHC (Paraffin sections) (IHC-P). GTX31936

GTX31936 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31936 Nephrin antibody.
Working concentration : 5 μg/ml

Anti-Nephrin antibody used in Western Blot (WB). GTX31936

GTX31936 WB Image

WB analysis of human kidney tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31936 Nephrin antibody.
Working concentration : 1 μg/ml

Anti-Nephrin antibody used in IHC (Paraffin sections) (IHC-P). GTX31936

GTX31936 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31936 Nephrin antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Li H et al. Aging (Albany NY) 2023; 15 (19) : 10291-10306 Dendrobium officinale polysaccharide decreases podocyte injury in diabetic nephropathy by regulating IRS-1/AKT signal and promoting mitophagy.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399