GeneTex
United States (US)

Nephrin antibody

GTX31936_3085_WB_20180221_18121410_995.jpg
GTX31936_1617_IHC-P_20180221_18121410_324.jpg
GTX31936_1618_IHC-P_20180221_18121410_392.jpg

Cat No. GTX31936

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

135 kDa. ( Note )

Positive Control

Human Kidney Tissue Lysate(GTX29995) , Human Kidney Tissue Slide

Specificity/Sensitivity

Nephrin antibody is human specific.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Nephrin antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Nephrin.The immunogen is located within amino acids 1100 - 1150 of Nephrin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nphs1, Nephrin,Cnf,Nphn,Nephrin,Nphs1

Background

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
GTX31936_3085_WB_20180221_18121410_995.jpg

GTX31936 WB Image

WB analysis of human kidney tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31936 Nephrin antibody.
Working concentration : 1 μg/ml

GTX31936_1617_IHC-P_20180221_18121410_324.jpg

GTX31936 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31936 Nephrin antibody.
Working concentration : 5 μg/ml

GTX31936_1618_IHC-P_20180221_18121410_392.jpg

GTX31936 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31936 Nephrin antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329