GeneTex
United States (US)

Nesprin 3 antibody

C14orf49-antibody-GTX87974-WB-1_18121409_120.jpg

Cat No. GTX87974

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

112 kDa. ( Note )

Specificity/Sensitivity

C14orf49 Antibody detects endogenous levels of total C14orf49 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human C14orf49.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Spectrin Repeat Containing Nuclear Envelope Family Member 3,C14Orf139,C14Orf49,Kash3,Linc00341,Ncrna00341,Net53,Nesp3,Syne3

Cellular Localization

Located at the rough reticulum endoplasmic (RER) in some cells.

Background

Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.

Database

DATA IMAGES
C14orf49-antibody-GTX87974-WB-1_18121409_120.jpg

GTX87974 WB Image

Western blot analysis of extracts from Jurkat cells, using C14orf49 Antibody. The lane on the right is treated with the synthesized peptide.

REFERENCE
Package List Price ($)
$ 349