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Nesprin 3 antibody

Orthogonal
Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974
Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974
Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974
Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974
Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974

Cat. No. GTX87974

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Nesprin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Nesprin 3 (169-218).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10726025

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C14orf49 , NET53 , Nesp3 , SYNE3 , spectrin repeat containing nuclear envelope family member 3 , Nesprin 3 , spectrin repeat containing, nuclear envelope family member 3

Cellular Localization

Nucleus outer membrane,Nucleus envelope,Rough endoplasmic reticulum

Database

Research Area

DATA IMAGES

Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974

GTX87974 WB Image

WB analysis of Jurkat cell lysates using GTX87974 Nesprin 3 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974

GTX87974 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2014.PMID: 24815916

Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974

GTX87974 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2014.PMID: 24815916

Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974

GTX87974 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2014.PMID: 24815916

Anti-Nesprin 3 antibody used in Western Blot (WB). GTX87974

GTX87974 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2014.PMID: 24815916

REFERENCE

Application Reference

Hobbs RP et al. Nat Genet 2015; 47 (8) : 933-8 Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes.
Application : WB
Reactivity : Human
Shaiken TE et al. Sci Rep 2014; 4 : 4923 Dissecting the cell to nucleus, perinucleus and cytosol.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399