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Nestin antibody [10C2]

Anti-Nestin antibody [10C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30671
Anti-Nestin antibody [10C2] used in Flow cytometry (FACS). GTX30671
Anti-Nestin antibody [10C2] used in IHC (Paraffin sections) (IHC-P). GTX30671
Anti-Nestin antibody [10C2] used in Western Blot (WB). GTX30671

Cat No. GTX30671

Host

Mouse

Clonality

Monoclonal

Clone Name

10C2

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, MS

Reactivity

Human, Mouse, Rat, Hamster
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:5000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
IHC-Fr 1:100 - 1:200
FACS 1 μg / 10⁶ cells
MS Assay dependent
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Specificity/Sensitivity

We do not recommend use of this product for Mouse,Rat,Rabbit.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nestin , Nbla00170

Background

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody [10C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30671

GTX30671 ICC/IF Image

ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2].
Green : primary antibody(left) and isotype control(right)
Blue : DAPI
Dilution : 1:50
Permibilization : 0.1% Triton X-100

Anti-Nestin antibody [10C2] used in Flow cytometry (FACS). GTX30671

GTX30671 FACS Image

FACS (Intracellular staining) analysis of 1 x 10⁶ CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/10⁶ cells

Anti-Nestin antibody [10C2] used in IHC (Paraffin sections) (IHC-P). GTX30671

GTX30671 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2].
Dilution : 1:500

Anti-Nestin antibody [10C2] used in Western Blot (WB). GTX30671

GTX30671 WB Image

WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.
Loading : 5μg
Dilution : 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369