WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.
Loading : 5μg
Dilution : 1:1000

IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2].
Dilution : 1:500

ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2].
Green : primary antibody(left) and isotype control(right)
Blue : DAPI
Dilution : 1:50
Permibilization : 0.1% Triton X-100

FACS (Intracellular staining) analysis of 1 x 10⁶ CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/10⁶ cells

The data was published in the journal PLoS One in 2015.PMID: 26720151