GeneTex
United States (US)

Nestin antibody [10C2]

GTX30671_234_ICC-IF_18121410_276.jpg
GTX30671_104_FACS_18121410_219.jpg
GTX30671_570_IHC-P_18121410_178.jpg
GTX30671_1471_WB_18121410_221.jpg

Cat No. GTX30671

Host Mouse
Clonality Monoclonal
Clone Name 10C2
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, MS
Reactivity Human, Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:5000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
IHC-Fr 1:100 - 1:200
FACS 1 μg / 10⁶ cells
MS Assay dependent
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Specificity/Sensitivity

This product does not react with Mouse,Rat,Rabbit.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.40 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nestin,Nbla00170,Nes

Background

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX30671_234_ICC-IF_18121410_276.jpg

GTX30671 ICC/IF Image

ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2].
Green : primary antibody(left) and isotype control(right)
Blue : DAPI
Dilution : 1:50
Permibilization : 0.1% Triton X-100

GTX30671_104_FACS_18121410_219.jpg

GTX30671 FACS Image

FACS (Intracellular staining) analysis of 1 x 10⁶ CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/10⁶ cells

GTX30671_570_IHC-P_18121410_178.jpg

GTX30671 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2].
Dilution : 1:500

GTX30671_1471_WB_18121410_221.jpg

GTX30671 WB Image

WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.
Loading : 5μg
Dilution : 1:1000

REFERENCE
Package List Price ($)
$ 329