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Nestin antibody [1A9G12]

Anti-Nestin antibody [1A9G12] used in ELISA (ELISA). GTX60677
Anti-Nestin antibody [1A9G12] used in Flow cytometry (FACS). GTX60677
Anti-Nestin antibody [1A9G12] used in Western Blot (WB). GTX60677

Cat. No. GTX60677

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1A9G12

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all Nestin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NESTIN (AA: 419-588 ) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nestin , Nbla00170

Background

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody [1A9G12] used in ELISA (ELISA). GTX60677

GTX60677 ELISA Image

ELISA analysis of antigen using GTX60677 Nestin antibody [1A9G12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Nestin antibody [1A9G12] used in Flow cytometry (FACS). GTX60677

GTX60677 FACS Image

FACS analysis of HeLa cells using GTX60677 Nestin antibody [1A9G12].
Green : Nestin
Purple : negative control

Anti-Nestin antibody [1A9G12] used in Western Blot (WB). GTX60677

GTX60677 WB Image

WB analysis of human Nestin recombinant protein using GTX60677 Nestin antibody [1A9G12].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409