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Nestin antibody [2C1.3A11]

Cat No. GTX18102

Host

Mouse

Clonality

Monoclonal

Clone Name

2C1.3A11

Isotype

IgG1

Application

WB, ICC/IF, FACS, IP, IHC

Reactivity

Human
Package
100 μl ($439)

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at 1:1000-1:5000
For ICC/IF: Use at 1:100-1:500.FACS:1:200
Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

177 kDa. ( Note )

Specificity/Sensitivity

Human nestin with no cross- reactivity to rodent

PROPERTIES

Form

Liquid

Buffer

PBS + 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 1464 and 1614 of human Nestin.

Purification

Protein G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Nestin , Nbla00170 , Nes

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 439