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Nestin antibody [2Q178]

Anti-Nestin antibody [2Q178] used in Immunohistochemistry (IHC). GTX26142
Anti-Nestin antibody [2Q178] used in IHC (Paraffin sections) (IHC-P). GTX26142

Cat. No. GTX26142

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2Q178

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Mouse, Rat, Dog, Hamster, Chicken
Package
50 μg ($429)
See all Nestin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:20-1:200
IHC-P 1:20-1:200
IHC-Fr 1:20-1:200
IHC Assay dependent

Note :

ICC/IF
Fix cells in 4% paraformaldehyde buffered with 50mM sodium borate, pH 9.5

IHC-P
Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM.

IHC-Fr
Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM.
Not tested in other applications.

Calculated MW

209 kDa. ( Note )

Product Note

We do not recommend use of this product for Human samples.

PROPERTIES

Form

Liquid

Buffer

100mM Tris-Glycine, 150mM NaCl

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Nestin purified from embryonic rat spinal cord.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_370407

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

intermediate filament protein specifically expressed in neuroepithelial stem cells; may play a role in neural cell differentiation [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody [2Q178] used in Immunohistochemistry (IHC). GTX26142

GTX26142 IHC Image

IHC analysis of mouse brain tissue sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin using GTX26142 Nestin antibody [2Q178].
Blue : Nissl (rough ER)
Red : nestin (Primary antibody)
Green : lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes)

Anti-Nestin antibody [2Q178] used in IHC (Paraffin sections) (IHC-P). GTX26142

GTX26142 IHC-P Image

IHC-P analysis of mouse pancreas tissue using GTX26142 Nestin antibody [2Q178].Immunoreactivity is detected in the islets of Langerhans.
Antigen retrieval : Citrate buffer, pH 6.0

REFERENCE

Application Reference

Berenice M?rquez-Valadez et al. Front. Neurosci. 2019; (Epub) The Systemic Administration of the Histamine H1 Receptor Antagonist/Inverse Agonist.
Application : IHC-P
Reactivity : Rat
Hsieh FY et al. Biomaterials 2018; (Epub) Non-viral delivery of an optogenetic tool into cells with self-healing hydrogel.
Application : ICC/IF
Reactivity : Mouse
Huang CT et al. Journal of Materials Chemistry B 2017; (44) A graphene–polyurethane composite hydrogel as a potential bioink for 3D bioprinting and differentiation of neural stem cells.
Application : ICC/IF
Reactivity : Mouse
Trias E et al. JCI Insight 2017; 2 (20) Evidence for mast cells contributing to neuromuscular pathology in an inherited model of ALS.
Application : IHC-P
Reactivity : Rat
Han HW et al. Colloids Surf B Biointerfaces 2017; 158 : 527-538 Chitosan derived co-spheroids of neural stem cells and mesenchymal stem cells for neural regeneration.
Application : ICC/IF
Reactivity : Mouse
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 429