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Nestin antibody [2Q178]

Anti-Nestin antibody [2Q178] used in IHC (Paraffin sections) (IHC-P). GTX26142
Anti-Nestin antibody [2Q178] used in Immunohistochemistry (IHC). GTX26142

Cat No. GTX26142

Host

Mouse

Clonality

Monoclonal

Clone Name

2Q178

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

Mouse, Rat, Dog, Hamster, Chicken
Package
50 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:20-1:200
IHC-P 1:20-1:200
IHC-Fr 1:20-1:200
IHC Assay dependent

Note :

ICC/IF
Fix cells in 4% paraformaldehyde buffered with 50mM sodium borate, pH 9.5

IHC-P
Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM.

IHC-Fr
Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM.

Not tested in other applications.

Calculated MW

209 kDa. ( Note )

Specificity/Sensitivity

We do not recommend use of this product for Human.

PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M sodium chloride pH 7.4, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Nestin purified from embryonic rat spinal cord.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

intermediate filament protein specifically expressed in neuroepithelial stem cells; may play a role in neural cell differentiation [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody [2Q178] used in IHC (Paraffin sections) (IHC-P). GTX26142

GTX26142 IHC-P Image

IHC-P analysis of mouse pancreas tissue using GTX26142 Nestin antibody [2Q178].Immunoreactivity is detected in the islets of Langerhans.
Antigen retrieval : Citrate buffer, pH 6.0

Anti-Nestin antibody [2Q178] used in Immunohistochemistry (IHC). GTX26142

GTX26142 IHC Image

IHC analysis of mouse brain tissue sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin using GTX26142 Nestin antibody [2Q178].
Blue : Nissl (rough ER)
Red : nestin (Primary antibody)
Green : lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes)

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 359