Nestin antibody [GT623]

Name: Nestin antibody [GT623]
Brand: GeneTex
SKU: GTX630201
Price: 419.00 USD

Cat. No. GTX630201

GTX630201 WB Image

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX630201 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX630201 ICC/IF Image

Nestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.
Green: Nestin stained by Nestin antibody [GT623] (GTX630201) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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This Nestin antibody [GT623] (GTX630201) is a mouse monoclonal antibody validated for detection of Nestin by WB and ICC/IF. Reactivity has been shown in Human. This product is cited in 18 published studies.

Host

Mouse
Clonality

Monoclonal
Clone Name

GT623
Isotype

IgG1
Applications

WB ICC/IF
Reactivity

Human

Summary

Nestin antibody recognizes nestin, a type-IV intermediate filament protein that migrates at ~240 kDa on western blot. Nestin is an established marker of mesodermal and neuroectodermal fates, and is expressed in neural stem cells. It is also enriched in endothelial cells, regardless of their proliferative state.