GeneTex
United States (US)

Nestin antibody [GT623]

Comparison
GTX630201_41512_20161109_IFA_18121411_801.jpg
GTX630201_41512_20171229_WB_18121411_929.jpg
GTX630201_41512_20161222_WB_competitor_watermark_18121411_944.jpg

Cat No. GTX630201

Host Mouse
Clonality Monoclonal
Clone Name GT623
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Positive Control

SH-SY-5Y , SK-N-SH , IMR32
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Nestin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nestin Antibody, NES Antibody, Nbla00170 Antibody

Background

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX630201_41512_20161109_IFA_18121411_801.jpg

GTX630201 ICC/IF Image

Nestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin protein stained by Nestin antibody [GT623] (GTX630201) diluted at 1:250.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX630201_41512_20171229_WB_18121411_929.jpg

GTX630201 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX630201_41512_20161222_WB_competitor_watermark_18121411_944.jpg

GTX630201 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169