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Nestin antibody [GT935]

Anti-Nestin antibody [GT935] used in Western Blot (WB). GTX630202
Anti-Nestin antibody [GT935] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630202

Cat. No. GTX630202

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT935

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Nestin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Positive Control

SH-SY-5Y , SK-N-SH

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Nestin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2716635

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nestin , Nbla00170

Background

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody [GT935] used in Western Blot (WB). GTX630202

GTX630202 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT935] (GTX630202) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nestin antibody [GT935] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630202

GTX630202 ICC/IF Image

Nestin antibody [GT935] detects Nestin protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin protein stained by Nestin antibody [GT935] (GTX630202) diluted at 1:100.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Chang CW et al. Stem Cell Res 2017; 27 : 21-24 Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations