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Nestin antibody [Rat-401 (4D4)]

Cat. No. GTX39577

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Rat-401 (4D4)

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
50 μg ($449)
See all Nestin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/20-1/200
IHC-Fr 1/20-1/200

Note :

IHC-P
We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM.

IHC-Fr
We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

TBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Nestin purified from embryonic rat spinal cord.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_11175711

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nestin

Background

intermediate filament protein specifically expressed in neuroepithelial stem cells; may play a role in neural cell differentiation [RGD, Feb 2006]

Database

Research Area

REFERENCE

Application Reference

Valle-Bautista R et al. Front Cell Dev Biol 2020; 8 : 564561 Impaired Cortical Cytoarchitecture and Reduced Excitability of Deep-Layer Neurons in the Offspring of Diabetic Rats.
Reactivity : Rat
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 449