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Nestin antibody [Rat-401 (4D4)]

Cat No. GTX39577

Host

Mouse

Clonality

Monoclonal

Clone Name

Rat-401 (4D4)

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
50 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/20-1/200
IHC-Fr 1/20-1/200

Note :

IHC-P
We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM.

IHC-Fr
We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM.

Not tested in other applications.

Calculated MW

209 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris buffered saline, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Nestin purified from embryonic rat spinal cord.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Nestin , Nes

Background

intermediate filament protein specifically expressed in neuroepithelial stem cells; may play a role in neural cell differentiation [RGD, Feb 2006]

Database

Research Area

REFERENCE

There are currently no references for Nestin antibody [Rat-401 (4D4)] (GTX39577). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Nestin antibody [Rat-401 (4D4)] (GTX39577). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339