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Nestin antibody

Anti-Nestin antibody used in Western Blot (WB). GTX00737
Anti-Nestin antibody used in IHC (Frozen sections) (IHC-Fr). GTX00737
Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00737
Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00737

Cat No. GTX00737

Host

Rabbit

Clonality

Polyclonal

Application

WB, ICC/IF, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
ICC/IF 1:300-1:1000
IHC-Fr 1:500-1:1000
Not tested in other applications.

Calculated MW

207 kDa. ( Note )

Specificity/Sensitivity

This product does not react with Human.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to the C-terminal 15 aa of mouse nestin

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Nestin antibody used in Western Blot (WB). GTX00737

GTX00737 WB Image

WB analysis of various samples using GTX00737 Nestin antibody.
Lane 1 : Mouse neucortex at E14.5
Lane 2 : Neural stem cells in culture prepared from E14.5 mouse neocortex
Lane 3 : Neurons in culture differentiated from neural stem cells
Dilution : 1:500
* : indicate non-specific bands.

Anti-Nestin antibody used in IHC (Frozen sections) (IHC-Fr). GTX00737

GTX00737 IHC-Fr Image

IHC-Fr analysis of mouse telencephalon tissue section using GTX00737 Nestin antibody.
Dilution : 1:500
Fixation : 4% PFA

Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00737

GTX00737 ICC/IF Image

ICC/IF analysis of mouse neuroepithelial cells using GTX00737 Nestin antibody.
Dilution : 1:300

Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00737

GTX00737 ICC/IF Image

ICC/IF analysis of Vn19 cells ( derived from mouse ependymoma) using GTX00737 Nestin antibody.
Red : Primary antibody
Blue : Hoechst 33342
Dilution : 1:1000
Fixation : Methanol-Acetone

REFERENCE

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REVIEW

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Package List Price ($)
$ 359