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Nestin antibody

Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00737
Anti-Nestin antibody used in Western Blot (WB). GTX00737
Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00737
Anti-Nestin antibody used in IHC (Frozen sections) (IHC-Fr). GTX00737

Cat. No. GTX00737

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μg ($399)
See all Nestin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF 1:300-1:1000
IHC-Fr 1:500-1:1000
Not tested in other applications.

Calculated MW

207 kDa. ( Note )

Product Note

We do not recommend use of this product for Human samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to the C-terminal 15 aa of mouse nestin

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nestin , AA166324 , C78523 , ESTM46 , Ifaprc2 , Marc2 , RC2

Database

Research Area

DATA IMAGES

Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00737

GTX00737 ICC/IF Image

ICC/IF analysis of mouse neuroepithelial cells using GTX00737 Nestin antibody.
Dilution : 1:300

Anti-Nestin antibody used in Western Blot (WB). GTX00737

GTX00737 WB Image

WB analysis of various samples using GTX00737 Nestin antibody.
Lane 1 : Mouse neucortex at E14.5
Lane 2 : Neural stem cells in culture prepared from E14.5 mouse neocortex
Lane 3 : Neurons in culture differentiated from neural stem cells
Dilution : 1:500
* : indicate non-specific bands.

Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00737

GTX00737 ICC/IF Image

ICC/IF analysis of Vn19 cells ( derived from mouse ependymoma) using GTX00737 Nestin antibody.
Red : Primary antibody
Blue : Hoechst 33342
Dilution : 1:1000
Fixation : Methanol-Acetone

Anti-Nestin antibody used in IHC (Frozen sections) (IHC-Fr). GTX00737

GTX00737 IHC-Fr Image

IHC-Fr analysis of mouse telencephalon tissue section using GTX00737 Nestin antibody.
Dilution : 1:500
Fixation : 4% PFA

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399