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Nestin antibody

Comparison
Anti-Nestin antibody used in Western Blot (WB). GTX133111
Anti-Nestin antibody used in Western Blot (WB). GTX133111
Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133111
Anti-Nestin antibody used in Western Blot (WB). GTX133111

Cat No. GTX133111

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all Nestin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

207 kDa. ( Note )

Positive Control

SK-N-SH , IMR32 , SH-SY-5Y , mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse Nestin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nestin,AA166324,C78523,ESTM46,Ifaprc2,Marc2,RC2

Background

May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division.

Database

Research Area

DATA IMAGES
Anti-Nestin antibody used in Western Blot (WB). GTX133111

GTX133111 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody (GTX133111) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Nestin antibody used in Western Blot (WB). GTX133111

GTX133111 WB Image

Various whole cell and tissue extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody (GTX133111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133111

GTX133111 ICC/IF Image

Nestin antibody detects Nestin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin stained by Nestin antibody (GTX133111) diluted at 1:500.
Red: TDP43, stained by TDP43 antibody [GT225] (GTX630196) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Nestin antibody used in Western Blot (WB). GTX133111

GTX133111 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody (GTX133111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169