1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.78mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse Nestin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division.
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody (GTX133111) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000.
*The competitor is not affiliated with GeneTex and does not endorse this product.
GTX133111 WB Image
Various whole cell and tissue extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody (GTX133111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX133111 ICC/IF Image
Nestin antibody detects Nestin protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nestin stained by Nestin antibody (GTX133111) diluted at 1:500.Red: TDP43, stained by TDP43 antibody [GT225] (GTX630196) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
GTX133111 WB Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody (GTX133111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package
List Price ($)
$ 319
$ 169
Literature
Poster - Neural Markers
Download the latest version of GeneTex's neural markers poster.