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GeneTex
United States (US)

Nestin antibody

Comparison
GTX133111_42592_20161222_WB_competitor_watermark_18121410_653.jpg
GTX133111_42592_20170629_WB_H_M_R_18121410_964.jpg
GTX133111_42592_20180329_ICC_IF_R_18121410_623.jpg
GTX133111_42592_20171229_WB_18121410_610.jpg

Cat No. GTX133111

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
See all Nestin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

207 kDa. ( Note )

Positive Control

SK-N-SH , IMR32 , SH-SY-5Y , mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse Nestin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nestin,Aa166324,C78523,Estm46,Ifaprc2,Marc2,Rc2,Nes

Background

May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division.

Database

Research Area

DATA IMAGES
GTX133111_42592_20161222_WB_competitor_watermark_18121410_653.jpg

GTX133111 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody (GTX133111) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX133111_42592_20170629_WB_H_M_R_18121410_964.jpg

GTX133111 WB Image

Various whole cell and tissue extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody (GTX133111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX133111_42592_20180329_ICC_IF_R_18121410_623.jpg

GTX133111 ICC/IF Image

Nestin antibody detects Nestin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin stained by Nestin antibody (GTX133111) diluted at 1:500.
Red: TDP43, stained by TDP43 antibody [GT225] (GTX630196) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GTX133111_42592_20171229_WB_18121410_610.jpg

GTX133111 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody (GTX133111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169