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Nestin antibody

Comparison
Anti-Nestin antibody used in Western Blot (WB). GTX133111
Anti-Nestin antibody used in Western Blot (WB). GTX133111
Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133111
Anti-Nestin antibody used in Western Blot (WB). GTX133111

Cat No. GTX133111

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Nestin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

207 kDa. ( Note )

Positive Control

SK-N-SH , IMR32 , SH-SY-5Y , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse Nestin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nestin , AA166324 , C78523 , ESTM46 , Ifaprc2 , Marc2 , RC2

Background

May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division.

Database

Research Area

DATA IMAGES

Anti-Nestin antibody used in Western Blot (WB). GTX133111

GTX133111 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody (GTX133111) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Nestin antibody used in Western Blot (WB). GTX133111

GTX133111 WB Image

Various whole cell and tissue extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody (GTX133111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133111

GTX133111 ICC/IF Image

Nestin antibody detects Nestin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin stained by Nestin antibody (GTX133111) diluted at 1:500.
Red: TDP43, stained by TDP43 antibody [GT225] (GTX630196) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Nestin antibody used in Western Blot (WB). GTX133111

GTX133111 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody (GTX133111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chang MC et al. Int Endod J 2020; (Epub) Effects of transforming growth factor-β1 on plasminogen activation in stem cells from the apical papilla: role of activating receptor-like kinase 5/Smad2 and mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signalling.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169