GeneTex
United States (US)

Nestin antibody

GTX30670_1470_WB_18121410_711.jpg
GTX30670_233_ICC-IF_18121410_867.jpg

Cat No. GTX30670

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Specificity/Sensitivity

This product does not react with Mouse,Rat,Rabbit.
PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Nestin protein (corresponding to residues 1464-1614). [UniProt# P48681]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nbla00170 antibody

Background

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX30670_1470_WB_18121410_711.jpg

GTX30670 WB Image

WB analysis of human fetal temporal lobe tissue lysate using GTX30670 Nestin antibody.
Lane 1 : 0.5 μg/ml
Lane 2 : 2 μg/ml
Dilution : 2 μg/ml
Loading : 30μg

GTX30670_233_ICC-IF_18121410_867.jpg

GTX30670 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX30670 Nestin antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

Package List Price ($)
$ 329