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Nestin antibody

Anti-Nestin antibody used in Western Blot (WB). GTX30670
Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30670

Cat No. GTX30670

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Dog
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Specificity/Sensitivity

We do not recommend use of this product for Mouse,Rat,Rabbit.

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Nestin protein (corresponding to residues 1464-1614). [UniProt# P48681]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nestin , Nbla00170

Background

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody used in Western Blot (WB). GTX30670

GTX30670 WB Image

WB analysis of human fetal temporal lobe tissue lysate using GTX30670 Nestin antibody.
Lane 1 : 0.5 μg/ml
Lane 2 : 2 μg/ml
Dilution : 2 μg/ml
Loading : 30μg

Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30670

GTX30670 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX30670 Nestin antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

REFERENCE

There are currently no references for Nestin antibody (GTX30670). Be the first to share your publications with this product.

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339