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Nestin antibody

Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30670
Anti-Nestin antibody used in Western Blot (WB). GTX30670

Cat. No. GTX30670

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Dog
Package
100 μl ($419)
See all Nestin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat,Rabbit samples.

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Nestin protein (corresponding to residues 1464-1614). [UniProt# P48681]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nestin , Nbla00170

Background

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30670

GTX30670 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX30670 Nestin antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

Anti-Nestin antibody used in Western Blot (WB). GTX30670

GTX30670 WB Image

WB analysis of human fetal temporal lobe tissue lysate using GTX30670 Nestin antibody.
Lane 1 : 0.5 μg/ml
Lane 2 : 2 μg/ml
Dilution : 2 μg/ml
Loading : 30μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419