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Nestin antibody

Anti-Nestin antibody used in IHC (Paraffin sections) (IHC-P). GTX39578
Anti-Nestin antibody used in IHC (Paraffin sections) (IHC-P). GTX39578
Anti-Nestin antibody used in Western Blot (WB). GTX39578

Cat. No. GTX39578

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Dog, Hamster, Macaque
Package
50 μg ($699)
See all Nestin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500-1/3,000
ICC/IF Assay dependent
IHC-P 5μg/ml
ELISA 1/20,000-1/80,000

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Not tested in other applications.

Calculated MW

207 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Nestin (aa1484-1500).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_11177943

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nestin , AA166324 , C78523 , ESTM46 , Ifaprc2 , Marc2 , RC2

Database

Research Area

DATA IMAGES

Anti-Nestin antibody used in IHC (Paraffin sections) (IHC-P). GTX39578

GTX39578 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX39578 Nestin antibody.

Anti-Nestin antibody used in IHC (Paraffin sections) (IHC-P). GTX39578

GTX39578 IHC-P Image

IHC-P analysis of human kidney tissue using GTX39578 Nestin antibody.

Anti-Nestin antibody used in Western Blot (WB). GTX39578

GTX39578 WB Image

The data was published in the journal Nutrients in 2015. PMID: 26043036

REFERENCE

Application Reference

Jiao Y et al. Nutrients 2015; 7 (6) : 4383-402 Resveratrol Inhibits the Invasion of Glioblastoma-Initiating Cells via Down-Regulation of the PI3K/Akt/NF-κB Signaling Pathway.
Application : IHC-P
Reactivity : Mouse
Ma F et al. Oncol Rep 2014; 32 (3) : 1071-7 ZNF703 promotes tumor cell proliferation and invasion and predicts poor prognosis in patients with colorectal cancer.
Application : ICC/IF
Reactivity : Human
Suarez-Pereira I et al. Hippocampus 2014; Adult newborn neurons are involved in learning acquisition and long-term memory formation: The distinct demands on temporal neurogenesis of different cognitive tasks.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 699