GeneTex
United States (US)

Netrin G1 antibody [N2C1], Internal

GTX115637_40310_WB_18121410_286.jpg
GTX115637_40310_IHC_M_18121410_466.jpg
GTX115637_40310_WB_M_brain_18121410_467.jpg

Cat No. GTX115637

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

HeLa , HepG2 , HCT116 , mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Netrin G1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Netrin G1,Lmnt1,Ntng1

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor

Background

Netrin G1 (NTNG1) belongs to a conserved family of proteins that act as axon guidance cues during vertebrate nervous system development (Nakashiba et al., 2000 [PubMed 10964959]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX115637_40310_WB_18121410_286.jpg

GTX115637 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX115637 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115637_40310_IHC_M_18121410_466.jpg

GTX115637 IHC-P Image

NTNG1 antibody [N2C1], Internal detects NTNG1 protein at on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NTNG1 antibody [N2C1], Internal (GTX115637) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115637_40310_WB_M_brain_18121410_467.jpg

GTX115637 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX115637 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169