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Netrin G1 antibody [N2C1], Internal

Anti-Netrin G1 antibody [N2C1], Internal used in Western Blot (WB). GTX115637
Anti-Netrin G1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX115637
Anti-Netrin G1 antibody [N2C1], Internal used in Western Blot (WB). GTX115637

Cat. No. GTX115637

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Netrin G1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

HepG2 , Mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Netrin G1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10625511

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

netrin G1 , Lmnt1

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor

Background

Netrin G1 (NTNG1) belongs to a conserved family of proteins that act as axon guidance cues during vertebrate nervous system development (Nakashiba et al., 2000 [PubMed 10964959]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Netrin G1 antibody [N2C1], Internal used in Western Blot (WB). GTX115637

GTX115637 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX115637 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Netrin G1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX115637

GTX115637 IHC-P Image

NTNG1 antibody [N2C1], Internal detects NTNG1 protein at on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NTNG1 antibody [N2C1], Internal (GTX115637) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Netrin G1 antibody [N2C1], Internal used in Western Blot (WB). GTX115637

GTX115637 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX115637 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Luo X et al. Brain Struct Funct 2019; (Epub) Transcriptomic profile of the subiculum-projecting VIP GABAergic neurons in the mouse CA1 hippocampus.
Application : IHC
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169