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Netrin G1 ligand antibody [N1N3]

Anti-Netrin G1 ligand antibody [N1N3] used in Western Blot (WB). GTX121508
Anti-Netrin G1 ligand antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX121508
Anti-Netrin G1 ligand antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121508
Anti-Netrin G1 ligand antibody [N1N3] used in Western Blot (WB). GTX121508

Cat. No. GTX121508

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Netrin G1 ligand products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

MCF-7 , mouse brain , rat brain

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Netrin G1 ligand. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721992

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich repeat containing 4C , NGL-1 , NGL1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

NGL1 is a specific binding partner for netrin G1 (NTNG1; MIM 608818), which is a member of the netrin family of axon guidance molecules (Lin et al., 2003 [PubMed 14595443]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Netrin G1 ligand antibody [N1N3] used in Western Blot (WB). GTX121508

GTX121508 WB Image

Sample (30 μg of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX121508 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Netrin G1 ligand antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX121508

GTX121508 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Netrin G1 ligand(GTX121508) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Netrin G1 ligand antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121508

GTX121508 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using Netrin G1 ligand(GTX121508) antibody at 1:500 dilution.

Anti-Netrin G1 ligand antibody [N1N3] used in Western Blot (WB). GTX121508

GTX121508 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Netrin 1 antibody (GTX121508) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Raghavan KS et al. Cancer Res Commun 2022; 2 (9) : 1017-1036 NetrinG1(+) cancer-associated fibroblasts generate unique extracellular vesicles that support the survival of pancreatic cancer cells under nutritional stress.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169