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NeuN antibody [2Q158]

Anti-NeuN antibody [2Q158] used in IHC (Paraffin sections) (IHC-P). GTX30773
Anti-NeuN antibody [2Q158] used in IHC (Paraffin sections) (IHC-P). GTX30773
Anti-NeuN antibody [2Q158] used in IHC (Paraffin sections) (IHC-P). GTX30773
Anti-NeuN antibody [2Q158] used in IHC (Frozen sections) (IHC-Fr). GTX30773
Anti-NeuN antibody [2Q158] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30773
Anti-NeuN antibody [2Q158] used in IHC (Paraffin sections) (IHC-P). GTX30773

Cat. No. GTX30773

Reference 34

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2Q158

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Rabbit, Dog, Crocodile
Package
250 μg ($439)
See all NeuN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:10-1:100
IHC-P 1:100
IHC-Fr Assay dependent

Note :

ICC/IF
Neurons in culture should be permeabilized with 0.1% Triton X-100.

IHC-P
Antigen retrieval : Citrate buffer, pH 6.0
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M PBS pH7.6, 0.25M NaCl

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Purified cell nuclei from mouse brain

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_1949456

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus,Cytoplasm

Database

Research Area

DATA IMAGES

Anti-NeuN antibody [2Q158] used in IHC (Paraffin sections) (IHC-P). GTX30773

GTX30773 IHC-P Image

IHC-P analysis of rat cerebellum tissue using GTX30773 NeuN antibody [2Q158].Immunoreactivity is seen as nuclear staining in the neurons in the granular layer.
Antigen retireval : Citrate Buffer, pH 6.0
Dilution : 1:100

Anti-NeuN antibody [2Q158] used in IHC (Paraffin sections) (IHC-P). GTX30773

GTX30773 IHC-P Image

IHC-P analysis of mouse brain tissue (dentate gyrus and subventricular zone) using GTX30773 NeuN antibody [2Q158].
Red : Primary antibody
Green : BrdU
Antigen retireval : Citrate Buffer, pH 6.0

Anti-NeuN antibody [2Q158] used in IHC (Paraffin sections) (IHC-P). GTX30773

GTX30773 IHC-P Image

IHC-P analysis of mouse brain tissue (dentate gyrus and subventricular zone) using GTX30773 NeuN antibody [2Q158].
Red : Primary antibody
Green : BrdU

Anti-NeuN antibody [2Q158] used in IHC (Frozen sections) (IHC-Fr). GTX30773

GTX30773 IHC-Fr Image

The data was published in the journal Mol Pain in 2015.PMID: 26255228

Anti-NeuN antibody [2Q158] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30773

GTX30773 ICC/IF Image

The data was published in the journal F1000Res in 2014. PMID: 25309736

Anti-NeuN antibody [2Q158] used in IHC (Paraffin sections) (IHC-P). GTX30773

GTX30773 IHC-P Image

The data was published in the journal Evodevo in 2013. PMID: 23819918

REFERENCE

Application Reference

YN Huang et al. Cell Transplant 2024; 33 : 9636897241237049 Pomalidomide Improves Motor Behavioral Deficits and Protects Cerebral Cortex and Striatum Against Neurodegeneration Through a Reduction of Oxidative/Nitrosative Damages and Neuroinflammation After Traumatic Brain Injury
Huang TL et al. Chin Neurosurg J 2022; 8 (1) : 39 Thrombospondin-2 promotes the proliferation and migration of glioma cells and contributes to the progression of glioma.
Application : IHC-Fr
Reactivity : Human
Schaefers C et al. Stem Cells Int 2022; 2022 : 1320950 The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.
Application : ICC/IF
Reactivity : Human
Wu C et al. J Biol Chem 2022; 298 (10) : 102443 Ubiquitin ligase Triad1 promotes neurite outgrowth by inhibiting MDM2-mediated ubiquitination of the neuroprotective factor pleiotrophin.
Application : IHC-P
Reactivity : Rat
Jingbo Wei et al. Appl Bionics Biomech 2022; 2022 : 7871302 The Temporal and Spatial Changes of Th17, Tregs, and Related Cytokines in Epilepsy Lesions.
Application : IHC-Fr
Reactivity : Rat
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REVIEW

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Application Reference
YN Huang et al. Cell Transplant 2024; 33:9636897241237049 Pomalidomide Improves Motor Behavioral Deficits and Protects Cerebral Cortex and Striatum Against Neurodegeneration Through a Reduction of Oxidative/Nitrosative Damages and Neuroinflammation After Traumatic Brain Injury
Huang TL et al. Chin Neurosurg J 2022; 8 (1):39 Thrombospondin-2 promotes the proliferation and migration of glioma cells and contributes to the progression of glioma.
Application : IHC-Fr
Reactivity : Human
Schaefers C et al. Stem Cells Int 2022; 2022:1320950 The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.
Application : ICC/IF
Reactivity : Human
Wu C et al. J Biol Chem 2022; 298 (10):102443 Ubiquitin ligase Triad1 promotes neurite outgrowth by inhibiting MDM2-mediated ubiquitination of the neuroprotective factor pleiotrophin.
Application : IHC-P
Reactivity : Rat
Jingbo Wei et al. Appl Bionics Biomech 2022; 2022:7871302 The Temporal and Spatial Changes of Th17, Tregs, and Related Cytokines in Epilepsy Lesions.
Application : IHC-Fr
Reactivity : Rat
Xiang P et al. Cell Death Discov 2022; 8 (1):89 miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2.
Application : IHC-P
Reactivity : Rat
Zhang K et al. Neural Regen Res 2022; 17 (8):1814-1820 Reducing host aldose reductase activity promotes neuronal differentiation of transplanted neural stem cells at spinal cord injury sites and facilitates locomotion recovery.
Huizhi Fei et al. Front Cell Dev Biol 2021; 9:700854 CTRP1 Attenuates Cerebral Ischemia/Reperfusion Injury via the PERK Signaling Pathway.
Application : IHC-P
Reactivity : Rat
Chang CY et al. Int J Mol Sci 2020; 21 (4) WNT3A Promotes Neuronal Regeneration upon Traumatic Brain Injury.
Li H et al. Int J Biol Sci 2020; 16 (7):1107-1120 C-terminal binding proteins 1 and 2 in traumatic brain injury-induced inflammation and their inhibition as an approach for anti-inflammatory treatment.
Zhao M et al. Cell Mol Neurobiol 2020; (Epub) Elevated miR-29a Contributes to Axonal Outgrowth and Neurological Recovery After Intracerebral Hemorrhage via Targeting PTEN/PI3K/Akt Pathway.
Application : IHC-Fr
Reactivity : Rat
Lin CT et al. Elife 2020; 9 3,6'-dithiopomalidomide reduces neural loss, inflammation, behavioral deficits in brain injury and microglial activation.
Application : ICC/IF, IHC-P
Reactivity : Rat
Hsu WH et al. Aging (Albany NY) 2020; 12 (11):10556-10577 T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice.
Application : WB
Reactivity : Mouse
Tang W et al. J Neurosci Res 2020; 98 (8):1588-1604 Transient sublethal hypoxia in neonatal rats causes reduced dendritic spines, aberrant synaptic plasticity, and impairments in memory.
Application : IHC-Fr
Reactivity : Rat
He L et al. Brain Behav Immun 2020; Toll-like Receptor 7 Contributes to Neuropathic Pain by Activating NF-κB in Primary Sensory Neurons.
Application : IHC-Fr
Reactivity : Mouse
Zhang LN et al. Mol Med Rep 2019; 19 (3):1521-1528 Astrocytes enhance the tolerance of rat cortical neurons to glutamate excitotoxicity.
Hong Li et al. bioRxiv 2019; (Epub) C-terminal binding proteins 1 and 2 in traumatic brain injury-induced inflammation and their inhibition as an approach for anti-inflammatory treatment.
Application : IHC-Fr
Reactivity : Mouse
Sun L et al. J Neurosci 2019; (Epub) Contribution of DNMT1 to neuropathic pain genesis partially through epigenetically repressing Kcna2 in primary afferent neurons.
Li J et al. Conf Proc IEEE Eng Med Biol Soc 2018; 2018:3537-3540 The Influence of Environment Stimulation on Learning and Memory Function in Rats with Medication Chemotherapy.
Application : IHC-P
Reactivity : Rat
Dibue-Adjei M et al. Cell Physiol Biochem 2017; 44 (3):935-947 Cav2.3 (R-Type) Calcium Channels are Critical for Mediating Anticonvulsive and Neuroprotective Properties of * In Vivo.
Application : IHC-P
Reactivity : Mouse
Liu X et al. Rejuvenation Res 2017; (Epub) Astrocyte Autophagy Flux Protects Neurons Against Oxygen-Glucose Deprivation and Ischemic/Reperfusion Injury.
Application : ICC/IF
Reactivity : Mouse
Zera K et al. PLoS One 2017; 12 (10):e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
Jeong JH et al. Exp Ther Med 2016; 12 (5):3021-3028 Intermittent fasting is neuroprotective in focal cerebral ischemia by minimizing autophagic flux disturbance and inhibiting apoptosis.
Gao YH et al. Purinergic Signal 2016; (Epub) Effect of electroacupuncture on the cervicospinal P2X7 receptor/fractalkine/CX3CR1 signaling pathway in a rat neck-incision pain model.
Application : IHC-Fr
Reactivity : Rat
Liang L et al. Sci Rep 2016; 6:37704 G9a participates in nerve injury-induced Kcna2 downregulation in primary sensory neurons.
Application : IHC-Fr
Reactivity : Mouse
JEONG JH et al. Exp Ther Med 2016; 12:3021-3028 Intermittent fasting is neuroprotective in focal cerebral ischemia by minimizing autophagic flux disturbance and inhibiting apoptosis.
Application : IHC-P
Reactivity : Rat
Jiang J et al. Neuron 2016; 90 (3):535-50 Gain of Toxicity from ALS/FTD-Linked Repeat Expansions in C9ORF72 Is Alleviated by Antisense Oligonucleotides Targeting GGGGCC-Containing RNAs.
Application : IHC-P
Reactivity : Mouse
Qiu HQ et al. Mol Pain 2015; 11:46 Koumine enhances spinal cord 3α-hydroxysteroid oxidoreductase expression and activity in a rat model of neuropathic pain.
Telias M et al. J Neurosci 2015; 35 (46):15295-306 Functional Deficiencies in Fragile X Neurons Derived from Human Embryonic Stem Cells.
Application : ICC/IF
Reactivity : Human
Tang W et al. Int J Clin Exp Med 2015; 8 (4):5897-903 GSK3β expression and phosphorylation during neuronal maturation in the rat dorsal root ganglion.
Application : ICC/IF
Reactivity : Rat
Telias M et al. F1000Res 2014; 3:196 Electrical maturation of neurons derived from human embryonic stem cells.
Yla-Outinen L et al. J Tissue Eng Regen Med 2014; 8 (3):186-94 Three-dimensional growth matrix for human embryonic stem cell-derived neuronal cells.
Reactivity : Human
Di-Poi N et al. Evodevo 2013; 4 (1):19 Crocodylians evolved scattered multi-sensory micro-organs.
Application : IHC-P
Reactivity : Crocodile
Liang L et al. J Pain 2013; 14 (6):638-47 Protein kinase B/Akt is required for complete Freund's adjuvant-induced upregulation of Nav1.7 and Nav1.8 in primary sensory neurons.
Application : IHC-Fr
Reactivity : Rat
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 439