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NeuN antibody [HL2194]

Recombinant antibody
Anti-NeuN antibody [HL2194] used in IHC (Paraffin sections) (IHC-P). GTX638198
Anti-NeuN antibody [HL2194] used in IHC (Frozen sections) (IHC-Fr). GTX638198
Anti-NeuN antibody [HL2194] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638198
Anti-NeuN antibody [HL2194] used in IHC (Paraffin sections) (IHC-P). GTX638198

Cat. No. GTX638198

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2194

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NeuN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human NeuN. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding fox-1 homolog 3 , FOX-3 , FOX3 , HRNBP3 , NEUN

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

Database

Research Area

DATA IMAGES

Anti-NeuN antibody [HL2194] used in IHC (Paraffin sections) (IHC-P). GTX638198

GTX638198 IHC-P Image

NeuN antibody [HL2194] detects NeuN protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
NeuN stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody [HL2194] used in IHC (Frozen sections) (IHC-Fr). GTX638198

GTX638198 IHC-Fr Image

NeuN antibody [HL2194] detects NeuN protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: NeuN stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuN antibody [HL2194] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638198

GTX638198 ICC/IF Image

NeuN antibody [HL2194] detects NeuN protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NeuN stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:500.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuN antibody [HL2194] used in IHC (Paraffin sections) (IHC-P). GTX638198

GTX638198 IHC-P Image

NeuN antibody [HL2194] detects NeuN protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
NeuN stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations