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NeuN antibody [HL2550]

Recombinant antibody
Anti-NeuN antibody [HL2550] used in Western Blot (WB). GTX638922
Anti-NeuN antibody [HL2550] used in IHC (Frozen sections) (IHC-Fr). GTX638922
Anti-NeuN antibody [HL2550] used in IHC (Paraffin sections) (IHC-P). GTX638922
Anti-NeuN antibody [HL2550] used in IHC (Paraffin sections) (IHC-P). GTX638922
Anti-NeuN antibody [HL2550] used in Western Blot (WB). GTX638922
Anti-NeuN antibody [HL2550] used in Western Blot (WB). GTX638922

Cat. No. GTX638922

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2550

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NeuN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide of mouse NeuN

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding protein, fox-1 homolog (C. elegans) 3 , Fox-3 , Hrnbp3 , NeuN , Neuna60

Cellular Localization

Nucleus,Cytoplasm

Database

Research Area

DATA IMAGES

Anti-NeuN antibody [HL2550] used in Western Blot (WB). GTX638922

GTX638922 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuN antibody [HL2550] (GTX638922) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NeuN antibody [HL2550] used in IHC (Frozen sections) (IHC-Fr). GTX638922

GTX638922 IHC-Fr Image

NeuN antibody [HL2550] detects NeuN protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: NeuN stained by NeuN antibody [HL2550] (GTX638922) diluted at 1:100.
Antigen Retrieval: 4% paraformaldehyde

Anti-NeuN antibody [HL2550] used in IHC (Paraffin sections) (IHC-P). GTX638922

GTX638922 IHC-P Image

NeuN antibody [HL2550] detects NeuN protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
NeuN stained by NeuN antibody [HL2550] (GTX638922) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody [HL2550] used in IHC (Paraffin sections) (IHC-P). GTX638922

GTX638922 IHC-P Image

NeuN antibody [HL2550] detects NeuN protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
NeuN stained by NeuN antibody [HL2550] (GTX638922) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody [HL2550] used in Western Blot (WB). GTX638922

GTX638922 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NeuN antibody [HL2550] (GTX638922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NeuN antibody [HL2550] used in Western Blot (WB). GTX638922

GTX638922 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuN antibody [HL2550] (GTX638922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations