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NeuN antibody

Anti-NeuN antibody used in Western Blot (WB). GTX00837
Anti-NeuN antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX00837

Cat No. GTX00837

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Application

WB, ICC/IF, IHC, IHC (Free Floating)

Reactivity

Human, Mouse, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1,000
ICC/IF 1:5,000-10,000
IHC 1:5,000-10,000
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

IgY preparation, 5mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

N-terminal 100 amino acids of human Fox3 expressed in and purified from E. coli

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

FOX3,HRNBP3,NEUN,RBFOX3,RNA binding protein, fox1 homolog 3,NeuN,RNA binding fox-1 homolog 3

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

Database

Research Area

DATA IMAGES

Anti-NeuN antibody used in Western Blot (WB). GTX00837

GTX00837 WB Image

WB analysis of mouse brain nuclear fraction lysate using GTX00837 NeuN antibody. Two bands at 46 and 50kDa mark correspond to the two isoforms of the FOX3/NeuN protein.
Dilution : 1:1000

Anti-NeuN antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX00837

GTX00837 IHC (Free Floating) Image

IHC (Free Floating) analysis of PFA-fixed rat hippocampus tissue using GTX00837 NeuN antibody. The NeuN antibody stains the nuclei and distal perikarya of most neurons, while the IBA1 antibody specifically labels microglial cells.
Red : Primary antibody
Green : IBA1
Dilution : 1:5,000

REFERENCE

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REVIEW

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Package List Price ($)
$ 319