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NeuN antibody

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974
Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974
Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974
Anti-NeuN antibody used in Western Blot (WB). GTX132974
Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

Cat. No. GTX132974

Reference 18

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Fish
Package
100 μl ($399),
25 μl ($169)
See all NeuN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human NeuN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886793

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding fox-1 homolog 3 , FOX-3 , FOX3 , HRNBP3 , NEUN

Database

Research Area

DATA IMAGES

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).
Red: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250.
Green: Beta amyloid (1-42) stained by Beta amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
This image was provided courtesy of a customer review.

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974

GTX132974 IHC-Fr Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
NeuN stained by NeuN antibody (GTX132974) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
NeuN stained by NeuN antibody (GTX132974) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
NeuN stained by NeuN antibody (GTX132974) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
NeuN stained by NeuN antibody (GTX132974) diluted at 1:2500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974

GTX132974 ICC/IF Image

NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis.
Sample: Rat E18 primary cortical neuron, DIV 8 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Green: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
NeuN stained by NeuN antibody (GTX132974) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974

GTX132974 ICC/IF Image

NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NeuN antibody used in Western Blot (WB). GTX132974

GTX132974 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuN antibody (GTX132974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974

GTX132974 ICC/IF Image

NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
NeuN stained by NeuN antibody (GTX132974) diluted at 1:2500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974

GTX132974 IHC-Fr Image

NeuN antibody detects NeuN protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974

GTX132974 IHC-Fr Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
NeuN stained by NeuN antibody (GTX132974) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974

GTX132974 IHC-Fr Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human brain.
NeuN stained by NeuN antibody (GTX132974) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chang YJ et al. Sci Adv 2024; 10 (8) : eadj0347 Sulfated disaccharide protects membrane and DNA damages from arginine-rich dipeptide repeats in ALS.
L Xue et al. Neural Regen Res 2024; 19 (9) : 2027-2035 Transplantation of human placental chorionic plate-derived mesenchymal stem cells for repair of neurological damage in neonatal hypoxic-ischemic encephalopathy
Application : IHC-Fr
C Montillot et al. Neurobiol Dis 2023; : 106259 Characterization of Vps13b-mutant mice reveals neuroanatomical and behavioral phenotypes with females less affected
Application : IHC-P
Reactivity : Mouse
Liang MZ et al. Cell Biosci 2023; 13 (1) : 96 Timely expression of PGAM5 and its cleavage control mitochondrial homeostasis during neurite re-growth after traumatic brain injury.
Hayashi Y et al. Brain Behav Immun Health 2023; 30 : 100622 Microglia cause structural remodeling of noradrenergic axon in the trigeminal spinal subnucleus caudalis after infraorbital nerve injury in rats.
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REVIEW

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Application Reference
Chang YJ et al. Sci Adv 2024; 10 (8):eadj0347 Sulfated disaccharide protects membrane and DNA damages from arginine-rich dipeptide repeats in ALS.
L Xue et al. Neural Regen Res 2024; 19 (9):2027-2035 Transplantation of human placental chorionic plate-derived mesenchymal stem cells for repair of neurological damage in neonatal hypoxic-ischemic encephalopathy
Application : IHC-Fr
C Montillot et al. Neurobiol Dis 2023;:106259 Characterization of Vps13b-mutant mice reveals neuroanatomical and behavioral phenotypes with females less affected
Application : IHC-P
Reactivity : Mouse
Liang MZ et al. Cell Biosci 2023; 13 (1):96 Timely expression of PGAM5 and its cleavage control mitochondrial homeostasis during neurite re-growth after traumatic brain injury.
Hayashi Y et al. Brain Behav Immun Health 2023; 30:100622 Microglia cause structural remodeling of noradrenergic axon in the trigeminal spinal subnucleus caudalis after infraorbital nerve injury in rats.
Lu Y et al. CNS Neurosci Ther 2023; 29 (5):1254-1271 miR-26a-5p alleviates CFA-induced chronic inflammatory hyperalgesia through Wnt5a/CaMKII/NFAT signaling in mice.
Application : IHC-Fr
Reactivity : Mouse
Feng T et al. bioRxiv 2023; Loss of TMEM106B exacerbates Tau pathology and neurodegeneration in PS19 mice.
De-Fong Huang et al. 2022; 119 (33) Neuronal splicing regulator RBFOX3 mediates seizures via regulating Vamp1 expression preferentially in NPY-expressing GABAergic neurons.
Application : IHC-Fr
Reactivity : Mouse
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
Application : WB
Huang J et al. Eur J Neurosci 2021; 54 (6):6304-6321 Panax notoginseng saponin attenuates the hypoxic-ischaemic injury in neonatal rats by regulating the expression of neurotrophin factors.
Application : IHC-P
Reactivity : Rat
Xiong LL et al. Am J Chin Med 2021; 49 (3):677-703 Effect of Sutellarin on Neurogenesis in Neonatal Hypoxia-Ischemia Rat Model: Potential Mechanisms of Action.
CH Chang et al. Mol Ther Nucleic Acids 2020; 20:812-822 MicroRNA-206 Regulates Stress-Provoked Aggressive Behaviors in Post-weaning Social Isolation Mice
Application : IHC-Fr
Chuang HC et al. Part Fibre Toxicol 2020; 17 (1):59 Neuropathology changed by 3- and 6-months low-level PM2.5 inhalation exposure in spontaneously hypertensive rats.
Application : IHC-P
Reactivity : Rat
Ruiz-Palacios M et al. Fish Shellfish Immunol 2020; 106:161-166 Establishment of a brain cell line (SaB-1) from gilthead seabream and its application to fish virology.
Application : ICC/IF
Reactivity : Fish
Ruiz-Palacios M et al. Sci Total Environ 2020; 708:134821 Establishment of a brain cell line (FuB-1) from mummichog (Fundulus heteroclitus) and its application to fish virology, immunity and nanoplastics toxicology.
Application : ICC/IF
Wu Y et al. Neuroscience 2019; 417:70-80 Histone Deacetylase Inhibitor MS-275 Alleviates Postoperative Cognitive Dysfunction in Rats by Inhibiting Hippocampal Neuroinflammation.
Application : IHC-P
Reactivity : Rat
Jhan MK et al. J Leukoc Biol 2018; (Epub) Anti-TNF-α restricts dengue virus-induced neuropathy.
Application : IHC-Fr
Reactivity : Mouse
Tang FR et al. Physiol Behav 2017; 175:37-46 Radioprotective effect of ursolic acid in radiation-induced impairment of neurogenesis, learning and memory in adolescent BALB/c mouse.
Application : IHC-Fr
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169