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NeuN antibody

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974
Anti-NeuN antibody used in Western Blot (WB). GTX132974
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974
Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974
Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974

Cat No. GTX132974

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

mouse Retina , mouse brain , rat brain

Predict Reactivity

Human, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human NeuN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rna Binding Fox-1 Homolog 3,Fox-3,Fox3,Hrnbp3,Neun,Rbfox3

Database

DATA IMAGES
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX132974

GTX132974 IHC-P Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).
Red: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250.
Green: Beta amyloid (1-42) stained by Beta amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
This image was provided courtesy of a customer review.

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974

GTX132974 IHC-Fr Image

NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuN antibody used in Western Blot (WB). GTX132974

GTX132974 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuN antibody (GTX132974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974

GTX132974 IHC-Fr Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974

GTX132974 ICC/IF Image

NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132974

GTX132974 ICC/IF Image

NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis.
Sample: Rat E18 primary cortical neuron, DIV 8 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Green: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974

GTX132974 IHC-Fr Image

NeuN antibody detects NeuN protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX132974

GTX132974 IHC-Fr Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169