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NeuN antibody

Anti-NeuN antibody used in Western Blot (WB). GTX133127
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX133127
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX133127
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX133127

Cat No. GTX133127

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all NeuN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Mouse NeuN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA binding protein, fox-1 homolog (C. elegans) 3 , Fox-3 , Hrnbp3 , NeuN , Neuna60

Cellular Localization

Nucleus , Cytoplasm

Background

RNA-binding protein that regulates alternative splicing events.

Database

Research Area

DATA IMAGES

Anti-NeuN antibody used in Western Blot (WB). GTX133127

GTX133127 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuN antibody (GTX133127) diluted at 1:1000.

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX133127

GTX133127 IHC-P Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: NeuN protein stained by NeuN antibody (GTX133127) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX133127

GTX133127 IHC-Fr Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NeuN protein stained by NeuN antibody (GTX133127) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX133127

GTX133127 IHC-Fr Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: NeuN protein stained by NeuN antibody (GTX133127) diluted at 1:250.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

REFERENCE

Application Reference

Ho MR et al. Sci Rep 2017; 7 (1) : 6910 Blockade of dengue virus infection and viral cytotoxicity in neuronal cells in vitro and in vivo by targeting endocytic pathways.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169