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NeuN antibody

Anti-NeuN antibody used in Western Blot (WB). GTX16208
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX16208
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX16208

Cat. No. GTX16208

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat, Bovine, Chicken, Pig, Horse
Package
100 μl ($399)
See all NeuN products

APPLICATION

Application Note

WB:1:5,000-1:10,000. IF/IHC 1:500-1:1,000.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fragment corresponding to Human NeuN aa 1-100 (N terminal)

Purification

Unpurified
Whole antiserum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding fox-1 homolog 3 , FOX-3 , FOX3 , HRNBP3 , NEUN

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

Database

Research Area

DATA IMAGES

Anti-NeuN antibody used in Western Blot (WB). GTX16208

GTX16208 WB Image

Western blot of rat brain homogenates stained with GTX16208. Antibody binds closely spaced 48 and 46kDa bands.

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX16208

GTX16208 IHC-Fr Image

Paraformaldehyde fixed frozen section of adult rat cortex stained with GTX16208 in red and counterstained for DNA in blue. Note that GTX16208 stains neuronal nuclei and distal perikarya. These antibodies do not bind to the nuclei of perikarya of non-neuronal cells, so that they can be used to identify neurons.

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX16208

GTX16208 IHC-Fr Image

Paraformaldehyde fixed frozen section of adult rat brain stem stained with NeuN antibody in red and counterstained for MAP2 in green. DNA is shown in blue. Note that NeuN antibody stains neuronal nuclei and distal perikarya and that the MAP2 antibody stains the dendrites extending from these cells. These antibodies do not bind to the nuclei of perikarya of any non-neuronal cells, so that they can be used to identify and quantify neurons.

REFERENCE

Application Reference

YN Huang et al. Cell Transplant 2024; 33 : 9636897241237049 Pomalidomide Improves Motor Behavioral Deficits and Protects Cerebral Cortex and Striatum Against Neurodegeneration Through a Reduction of Oxidative/Nitrosative Damages and Neuroinflammation After Traumatic Brain Injury
Feng L et al. iScience 2023; 26 (11) : 108037 Developing a human iPSC-derived three-dimensional myelin spheroid platform for modeling myelin diseases.
Manet C et al. J Neuroinflammation 2022; 19 (1) : 307 Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response.
Application : ICC/IF
Reactivity : Mouse
Jianfei Chao et al. iScience 2022; 25 (6) : 104391 Therapeutic development for Canavan disease using patient iPSCs introduced with the wild-type ASPA gene.
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Package List Price ($)
$ 399