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NeuN antibody

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX32746
Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32746
Anti-NeuN antibody used in Western Blot (WB). GTX32746
Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32746
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX32746

Cat No. GTX32746

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RBFOX3 (NP_001076045.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA binding fox-1 homolog 3 , FOX-3 , FOX3 , HRNBP3 , NEUN

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

Database

Research Area

DATA IMAGES

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX32746

GTX32746 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32746 NeuN antibody.
Dilution : 1:100

Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32746

GTX32746 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32746 NeuN antibody.
Blue : DAPI
Dilution : 1:100

Anti-NeuN antibody used in Western Blot (WB). GTX32746

GTX32746 WB Image

WB analysis of various sample lysates using GTX32746 NeuN antibody. The signal was developed with ECL plus-Enhanced.
Loading : 25ug per lane
Dilution : 1:1000

Anti-NeuN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32746

GTX32746 ICC/IF Image

ICC/IF analysis of U-251MG cells using GTX32746 NeuN antibody.
Blue : DAPI
Dilution : 1:100

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX32746

GTX32746 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32746 NeuN antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329