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Neuregulin-1 antibody [C1C3]

Anti-Neuregulin-1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101116
Anti-Neuregulin-1 antibody [C1C3] used in Western Blot (WB). GTX101116
Anti-Neuregulin-1 antibody [C1C3] used in Western Blot (WB). GTX101116
Anti-Neuregulin-1 antibody [C1C3] used in Western Blot (WB). GTX101116

Cat. No. GTX101116

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all Neuregulin-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , mouse brain , rat brain

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Neuregulin-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175173

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neuregulin 1 , ARIA , GGF , GGF2 , HGL , HRG , HRG1 , HRGA , MST131 , MSTP131 , NDF , NRG1-IT2 , SMDF

Cellular Localization

Pro-neuregulin-1 , membrane-bound isoform: Cell membrane; Single-pass type I membrane protein , Neuregulin-1: Secreted , Isoform 8: Nucleus , Isoform 9: Secreted , Isoform 10: Membrane; Single-pass type I membrane protein

Background

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Neuregulin-1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101116

GTX101116 ICC/IF Image

Neuregulin-1 antibody [C1C3] detects Neuregulin-1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neuregulin-1 stained by Neuregulin-1 antibody [C1C3] (GTX101116) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Neuregulin-1 antibody [C1C3] used in Western Blot (WB). GTX101116

GTX101116 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody [C1C3] (GTX101116) diluted at 1:2000.

Anti-Neuregulin-1 antibody [C1C3] used in Western Blot (WB). GTX101116

GTX101116 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX101116 diluted at 1:2000

Anti-Neuregulin-1 antibody [C1C3] used in Western Blot (WB). GTX101116

GTX101116 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody [C1C3] (GTX101116) diluted at 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169