GeneTex
United States (US)

Neuregulin-1 antibody [C1C3]

GTX101116_40506_20151105_WB_M_brain_19011506_560.jpg
GTX101116_40506_20151105_WB_R_brain_19011506_139.jpg
GTX101116_40506_WB_19011506_217.jpg

Cat No. GTX101116

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , Mouse brain , Rat brain

Predict Reactivity

Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neuregulin 1,Aria,Ggf,Ggf2,Hgl,Hrg,Hrg1,Hrga,Mst131,Mstp131,Ndf,Nrg1-It2,Smdf,Nrg1

Cellular Localization

Pro-neuregulin-1 , membrane-bound isoform: Cell membrane; Single-pass type I membrane protein , Neuregulin-1: Secreted , Isoform 8: Nucleus , Isoform 9: Secreted , Isoform 10: Membrane; Single-pass type I membrane protein

Background

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101116_40506_20151105_WB_M_brain_19011506_560.jpg

GTX101116 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody [C1C3] (GTX101116) diluted at 1:2000.

GTX101116_40506_20151105_WB_R_brain_19011506_139.jpg

GTX101116 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody [C1C3] (GTX101116) diluted at 1:2000.

GTX101116_40506_WB_19011506_217.jpg

GTX101116 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX101116 diluted at 1:2000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169