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Neuregulin-1 antibody

Anti-Neuregulin-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101117
Anti-Neuregulin-1 antibody used in Western Blot (WB). GTX101117
Anti-Neuregulin-1 antibody used in Western Blot (WB). GTX101117
Anti-Neuregulin-1 antibody used in Western Blot (WB). GTX101117
Anti-Neuregulin-1 antibody used in Western Blot (WB). GTX101117

Cat No. GTX101117

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Human brain , Mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neuregulin 1,ARIA,GGF,GGF2,HGL,HRG,HRG1,HRGA,MST131,MSTP131,NDF,NRG1-IT2,SMDF

Cellular Localization

Pro-neuregulin-1 , membrane-bound isoform: Cell membrane; Single-pass type I membrane protein , Neuregulin-1: Secreted , Isoform 8: Nucleus , Isoform 9: Secreted , Isoform 10: Membrane; Single-pass type I membrane protein

Background

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Neuregulin-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101117

GTX101117 ICC/IF Image

Neuregulin-1 antibody detects Neuregulin-1 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neuregulin-1 protein stained by Neuregulin-1 antibody (GTX101117) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.

Anti-Neuregulin-1 antibody used in Western Blot (WB). GTX101117

GTX101117 WB Image

U87-MG whole cell extract and conditioned medium (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody (GTX101117) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Neuregulin-1 antibody used in Western Blot (WB). GTX101117

GTX101117 WB Image

Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody (GTX101117) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Neuregulin-1 antibody used in Western Blot (WB). GTX101117

GTX101117 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody (GTX101117) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Neuregulin-1 antibody used in Western Blot (WB). GTX101117

GTX101117 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX101117 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169