NeuroD1 antibody

Cat No. GTX133207

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Fr
Reactivity	Human, Rat
Package	100 μl ($379), 25 μl ($169)

See all NeuroD1 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

IMR32 , IMR32 nuclear extract

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse NeuroD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886865

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neurogenic differentiation 1 , BETA2 , BHF-1 , Nd1 , Neurod , bHLHa3

Cellular Localization

Cytoplasm , Nucleus

Background

Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.

Database

Gene ID: 18012 Neurod1
UniProt: Q60867 Neurod1

Research Area

DATA IMAGES

GTX133207 IHC-Fr Image

NeuroD1 antibody detects NeuroD1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: NeuroD1 stained by NeuroD1 antibody (GTX133207) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133207 WB Image

IMR32 whole cell and nuclear extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NeuroD1 antibody (GTX133207) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 379

25 μl

$ 169

Qty