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NeuroD1 antibody

Orthogonal
Anti-NeuroD1 antibody used in Western Blot (WB). GTX133207
Anti-NeuroD1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133207
Anti-NeuroD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133207

Cat. No. GTX133207

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all NeuroD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

IMR32 , IMR32 nuclear extract

Predict Reactivity

Mouse, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse NeuroD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886865

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurogenic differentiation 1 , BETA2 , BHF-1 , Nd1 , Neurod , bHLHa3

Cellular Localization

Cytoplasm , Nucleus

Background

Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.

Database

Research Area

DATA IMAGES

Anti-NeuroD1 antibody used in Western Blot (WB). GTX133207

GTX133207 WB Image

Orthogonal

IMR32 whole cell and nuclear extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NeuroD1 antibody (GTX133207) diluted at 1:1000.

Anti-NeuroD1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133207

GTX133207 IHC-Fr Image

NeuroD1 antibody detects NeuroD1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: NeuroD1 stained by NeuroD1 antibody (GTX133207) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuroD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133207

GTX133207 IHC-P Image

NeuroD1 antibody detects NeuroD1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
NeuroD1 stained by NeuroD1 antibody (GTX133207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169