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NeuroD1 antibody

Orthogonal
Anti-NeuroD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134069
Anti-NeuroD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134069
Anti-NeuroD1 antibody used in Western Blot (WB). GTX134069

Cat. No. GTX134069

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NeuroD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

IMR32 , IMR32 nuclear extract

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse NeuroD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887199

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurogenic differentiation 1 , BETA2 , BHF-1 , Nd1 , Neurod , bHLHa3

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-NeuroD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134069

GTX134069 ICC/IF Image

NeuroD1 antibody detects NeuroD1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NeuroD1 stained by NeuroD1 antibody (GTX134069) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuroD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134069

GTX134069 IHC-P Image

NeuroD1 antibody detects NeuroD1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded E10.5 mouse embryo.
NeuroD1 stained by NeuroD1 antibody (GTX134069) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuroD1 antibody used in Western Blot (WB). GTX134069

GTX134069 WB Image

Orthogonal

IMR32 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuroD1 antibody (GTX134069) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Drews HJ et al. Pharmaceuticals (Basel) 2021; 14 (2) Losartan Improves Memory, Neurogenesis and Cell Motility in Transgenic Alzheimer's Mice.
Application : WB
Reactivity : Mouse
Mansel C et al. Neurotoxicol Teratol 2019; 72 : 58-70 Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells.
Application : ICC/IF
Reactivity : Mouse
Drews HJ et al. Neurotherapeutics 2019; (Epub) Intranasal * Decreases Perivascular Beta Amyloid, Inflammation, and the Decline of Neurogenesis in Hypertensive Rats.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169