APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 5-10μg/ml |
|
Note :
IHC-P
Human Cerebral Cortex shows textured cytoplasm staining in the neuronal cell bodies.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89207-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_056493.3) and isoform 2 (NP_001191126.1).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-DFNRWHYEHQNRY, from the C Terminus of the protein sequence according to NP_056493.3; NP_001191126.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
neurobeachin , BCL8B , LYST2
Cellular Localization
Cytoplasm , Membrane
Background
This gene encodes a member of a large, diverse group of A-kinase anchor proteins that target the activity of protein kinase A to specific subcellular sites by binding to its type II regulatory subunits. Brain-specific expression and coat protein-like membrane recruitment of a highly similar protein in mouse suggest an involvement in neuronal post-Golgi membrane traffic. Mutations in this gene may be associated with a form of autism. This gene and its expression are frequently disrupted in patients with multiple myeloma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants may exist, but their full-length nature has not been determined.[provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
|
GTX89207 IHC-P Image
IHC-P analysis of human cerebral cortex using GTX89207 Neurobeachin antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
|
|
GTX89207 WB Image
WB analysis of 1) untransfected HEK293T cells 2) HEK293T cells transfected with mouse Neurobeachin using GTX89207 Neurobeachin antibody, C-term.
Dilution : 1μg/ml
|
REFERENCE
There are currently no references for Neurobeachin antibody, C-term (GTX89207). Be the first to share your publications with this product.
REVIEW
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