GeneTex
United States (US)

Neurofibromin antibody [McNFn27a]

KO/KD
GTX30208_1364_WB_18121410_979.jpg
GTX30208_540_IHC-P_18121410_368.jpg

Cat No. GTX30208

Host Mouse
Clonality Monoclonal
Clone Name McNFn27a
Isotype IgG1
Application WB, IHC-P, IP, ELISA
Reactivity Human, Mouse, Rat, Guinea pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 - 5 μg/ml
IHC-P 1:20 - 1:50
IP 1:10 - 1:500
ELISA 1:100 - 1:2000
Not tested in other applications.

Calculated MW

319 kDa. ( Note )

Specificity/Sensitivity

This is specific for full-length or truncated forms of neurofibromin.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A highly conserved N-terminal peptide, corresponding to amino acids 27-41 of human Neurofibromin 1. [UniProt# P21359]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofibromin 1,Nfns,Vrnf,Wss,Nf1

Cellular Localization

Cytoplasmic

Background

This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30208_1364_WB_18121410_979.jpg

GTX30208 WB Image

KO/KD

WB analysis of schwann cells from embryonic knockout mice, adult human nerves, and neonatal rat nerves using GTX30208 Neurofibromin antibody [McNFn27a].

GTX30208_540_IHC-P_18121410_368.jpg

GTX30208 IHC-P Image

IHC-P analysis of human brain tissue using GTX30208 Neurofibromin antibody [McNFn27a].
Dilution : 1:50
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)