GeneTex
United States (US)

Neurofibromin antibody [McNFn27b]

GTX15776_304_ICC-IF_19011519_768.jpg
GTX15776_302_ICC-IF_19011519_903.jpg
GTX15776_1037_IHC-P_19011519_232.jpg
GTX15776_305_ICC-IF_19011519_765.jpg
GTX15776_1038_IHC-P_19011519_671.jpg
GTX15776_1528_WB_19011519_886.jpg
GTX15776_303_ICC-IF_19011519_982.jpg

Cat No. GTX15776

Host Mouse
Clonality Monoclonal
Clone Name McNFn27b
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3 μg/ml
ICC/IF 1-2 μg/ml
IHC-P 5-10 μg/mL
IHC Assay dependent
Not tested in other applications.

Calculated MW

319 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofibromin 1,Nfns,Vrnf,Wss,Nf1

Cellular Localization

Nucleolus,Nucleus

Background

This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15776_304_ICC-IF_19011519_768.jpg

GTX15776 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15776 Neurofibromin antibody [McNFn27b]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

GTX15776_302_ICC-IF_19011519_903.jpg

GTX15776 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX15776 Neurofibromin antibody [McNFn27b]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

GTX15776_1037_IHC-P_19011519_232.jpg

GTX15776 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX15776 Neurofibromin antibody [McNFn27b].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX15776_305_ICC-IF_19011519_765.jpg

GTX15776 ICC/IF Image

ICC/IF analysis of U251 cells using GTX15776 Neurofibromin antibody [McNFn27b]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

GTX15776_1038_IHC-P_19011519_671.jpg

GTX15776 IHC-P Image

IHC-P analysis of human stomach tissue using GTX15776 Neurofibromin antibody [McNFn27b].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX15776_1528_WB_19011519_886.jpg

GTX15776 WB Image

WB analysis of whole cell extracts (30 µg lysate) of MCF-7 (Lane 1) and HepG2 (Lane 2) using GTX15776 Neurofibromin antibody [McNFn27b].
Dilution : 1-3 µg/ml

GTX15776_303_ICC-IF_19011519_982.jpg

GTX15776 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX15776 Neurofibromin antibody [McNFn27b]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE