GeneTex
United States (US)

Neurofibromin antibody

KO/KD
GTX28132_306_IHC-P_18121410_758.jpg
GTX28132_1635_WB_18121410_547.jpg

Cat No. GTX28132

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P 1:200 - 1:500
IHC-Fr 1:200 - 1:500
Not tested in other applications.

Calculated MW

319 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Serum, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An N-terminal peptide of Neurofibromin 1 (within amino acids 1-50). [UniProt# P21359]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofibromin 1,Nfns,Vrnf,Wss,Nf1

Cellular Localization

Cytoplasmic

Background

This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX28132_306_IHC-P_18121410_758.jpg

GTX28132 IHC-P Image

IHC-P analysis of maturing and the hypertrophic cartilage tissue using GTX28132 Neurofibromin antibody.

GTX28132_1635_WB_18121410_547.jpg

GTX28132 WB Image

KO/KD

WB analysis of (A) Neurofibromin knockout cell, (B) rat Schwann cell, and (C) human Schwann cell lysate using GTX28132 Neurofibromin antibody.