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Neurogenin 2 antibody

Anti-Neurogenin 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129258
Anti-Neurogenin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129258
Anti-Neurogenin 2 antibody used in Western Blot (WB). GTX129258
Anti-Neurogenin 2 antibody used in Western Blot (WB). GTX129258

Cat. No. GTX129258

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Neurogenin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

mouse embryonic midbrain , U87-MG , SK-N-SH

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Neurogenin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885944

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurogenin 2 , Atoh4 , Math4A , NGN2 , bHLHa8 , ngn-2

Cellular Localization

Nucleus

Background

Neurogenin-2 is a member of the neurogenin subfamily of basic helix-loop-helix (bHLH) transcription factor genes that play an important role in neurogenesis from migratory neural crest cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Neurogenin 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129258

GTX129258 IHC-Fr Image

Neurogenin 2 antibody detects Neurogenin 2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Neurogenin 2 protein stained by Neurogenin 2 antibody (GTX129258) diluted at 1:250.
Red: SOX2, stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Neurogenin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129258

GTX129258 ICC/IF Image

Neurogenin 2 antibody detects Neurogenin 2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neurogenin 2 stained by Neurogenin 2 antibody (GTX129258) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Neurogenin 2 antibody used in Western Blot (WB). GTX129258

GTX129258 WB Image

Neurogenin 2 antibody detects Neurogenin 2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Neurogenin 2 antibody (GTX129258) diluted by 1:2000.

Anti-Neurogenin 2 antibody used in Western Blot (WB). GTX129258

GTX129258 WB Image

Neurogenin 2 antibody detects Neurogenin 2 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with Neurogenin 2 antibody (GTX129258) diluted by 1:2000.

REFERENCE

Application Reference

Chen A et al. Front Mol Neurosci 2023; 16 : 1151162
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169