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Neurogenin 2 antibody

Anti-Neurogenin 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129258
Anti-Neurogenin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129258
Anti-Neurogenin 2 antibody used in Western Blot (WB). GTX129258
Anti-Neurogenin 2 antibody used in Western Blot (WB). GTX129258

Cat No. GTX129258

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , mouse embryonic midbrain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Neurogenin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neurogenin 2 , Atoh4 , Math4A , NGN2 , bHLHa8 , ngn-2

Cellular Localization

Nucleus

Background

Neurogenin-2 is a member of the neurogenin subfamily of basic helix-loop-helix (bHLH) transcription factor genes that play an important role in neurogenesis from migratory neural crest cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Neurogenin 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129258

GTX129258 IHC-Fr Image

Neurogenin 2 antibody detects Neurogenin 2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Neurogenin 2 protein stained by Neurogenin 2 antibody (GTX129258) diluted at 1:250.
Red: SOX2, stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Neurogenin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129258

GTX129258 ICC/IF Image

Neurogenin 2 antibody detects Neurogenin 2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neurogenin 2 stained by Neurogenin 2 antibody (GTX129258) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Neurogenin 2 antibody used in Western Blot (WB). GTX129258

GTX129258 WB Image

Neurogenin 2 antibody detects Neurogenin 2 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with Neurogenin 2 antibody (GTX129258) diluted by 1:2000.

Anti-Neurogenin 2 antibody used in Western Blot (WB). GTX129258

GTX129258 WB Image

Neurogenin 2 antibody detects Neurogenin 2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Neurogenin 2 antibody (GTX129258) diluted by 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169