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Neuroglobin antibody

Anti-Neuroglobin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54552
Anti-Neuroglobin antibody used in Western Blot (WB). GTX54552

Cat. No. GTX54552

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Neuroglobin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human NGB (NP_067080.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887862

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neuroglobin

Cellular Localization

Cytoplasm,Mitochondrion

Background

This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Neuroglobin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54552

GTX54552 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54552 Neuroglobin antibody.
Blue : DAPI

Anti-Neuroglobin antibody used in Western Blot (WB). GTX54552

GTX54552 WB Image

WB analysis of various sample lysates using GTX54552 Neuroglobin antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Gao Y et al. Front Pharmacol 2020; 11 : 555532 Dexmedetomidine Mediates Neuroglobin Up-Regulation and Alleviates the Hypoxia/Reoxygenation Injury by Inhibiting Neuronal Apoptosis in Developing Rats.
Application : WB
Reactivity : Rat
Gao Y et al. Life Sci 2019; : 116611 Dexmedetomidine protects hippocampal neurons against hypoxia/reoxygenation-induced apoptosis through activation HIF-1α/p53 signaling.
Application : WB
Reactivity : Rat
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399