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Neurogranin antibody, Internal

Anti-Neurogranin antibody, Internal used in Western Blot (WB). GTX88301
Anti-Neurogranin antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88301

Cat. No. GTX88301

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($419)
See all Neurogranin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

8 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88301-PEP).

Product Note

This antibody would recognize neurogranin precursor.

Predict Reactivity

Mouse, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KIKSGERGRKG, from the internal region of the protein sequence according to NP_006167.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurogranin , RC3 , hng

Background

Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Neurogranin antibody, Internal used in Western Blot (WB). GTX88301

GTX88301 WB Image

WB analysis of human brain (frontal cortex) lysate using GTX88301 Neurogranin antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Neurogranin antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88301

GTX88301 IHC-P Image

IHC-P analysis of human cortex using GTX88301 Neurogranin antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419