GeneTex
United States (US)

Neurogranin antibody, Internal

GTX88301_2428_WB_18122102_612.jpg
GTX88301_1014_IHC-P_18122102_524.jpg

Cat No. GTX88301

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

8 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88301-PEP).

Specificity/Sensitivity

This antibody would recognize neurogranin precursor.

Predict Reactivity

Mouse, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KIKSGERGRKG, from the internal region of the protein sequence according to NP_006167.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurogranin,Rc3,Hng,Nrgn

Background

Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88301_2428_WB_18122102_612.jpg

GTX88301 WB Image

WB analysis of human brain (frontal cortex) lysate using GTX88301 Neurogranin antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX88301_1014_IHC-P_18122102_524.jpg

GTX88301 IHC-P Image

IHC-P analysis of human cortex using GTX88301 Neurogranin antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml

Package List Price ($)
$ 329