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Neuroligin 1 antibody

Anti-Neuroligin 1 antibody used in Western Blot (WB). GTX133208
Anti-Neuroligin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133208

Cat No. GTX133208

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse Neuroligin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neuroligin 1,6330415N05Rik,BB179718,NL1,Nlg1,mKIAA1070

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Cell junction , synapse , synapse , postsynaptic cell membrane , postsynaptic density

Background

Neuronal cell surface protein thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. Seems to play role in formation or maintenance of synaptic junctions. In vitro, triggers the de novo formation of presynaptic structures (By similarity). May be involved in specification of excitatory synapses.

Database

Research Area

DATA IMAGES
Anti-Neuroligin 1 antibody used in Western Blot (WB). GTX133208

GTX133208 WB Image

Various tissue extracts (75 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuroligin 1 antibody (GTX133208) diluted at 1:1000.

Anti-Neuroligin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133208

GTX133208 ICC/IF Image

Neuroligin 1 antibody detects Neuroligin 1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neuroligin 1 protein stained by Neuroligin 1 antibody (GTX133208) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169