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Neuropeptide Y antibody

Anti-Neuropeptide Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133138
Anti-Neuropeptide Y antibody used in Western Blot (WB). GTX133138
Anti-Neuropeptide Y antibody used in IHC (Frozen sections) (IHC-Fr). GTX133138
Anti-Neuropeptide Y antibody used in IHC (Paraffin sections) (IHC-P). GTX133138

Cat No. GTX133138

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Predict Reactivity

Human, Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Neuropeptide Y. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neuropeptide Y , 0710005A05Rik

Cellular Localization

Secreted

Background

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

Database

Research Area

DATA IMAGES

Anti-Neuropeptide Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133138

GTX133138 ICC/IF Image

Neuropeptide Y antibody detects Neuropeptide Y protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neuropeptide Y stained by Neuropeptide Y antibody (GTX133138) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Neuropeptide Y antibody used in Western Blot (WB). GTX133138

GTX133138 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Neuropeptide Y antibody (GTX133138) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Neuropeptide Y antibody used in IHC (Frozen sections) (IHC-Fr). GTX133138

GTX133138 IHC-Fr Image

Neuropeptide Y antibody detects Neuropeptide Y protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Neuropeptide Y stained by Neuropeptide Y antibody (GTX133138) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Neuropeptide Y antibody used in IHC (Paraffin sections) (IHC-P). GTX133138

GTX133138 IHC-P Image

Neuropeptide Y antibody detects Neuropeptide Y protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Neuropeptide Y antibody (GTX133138) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169