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Neuropeptide Y antibody

Anti-Neuropeptide Y antibody used in IHC (Frozen sections) (IHC-Fr). GTX133138
Anti-Neuropeptide Y antibody used in Western Blot (WB). GTX133138
Anti-Neuropeptide Y antibody used in IHC (Paraffin sections) (IHC-P). GTX133138
Anti-Neuropeptide Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133138

Cat. No. GTX133138

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Neuropeptide Y products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Predict Reactivity

Human, Rabbit, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Neuropeptide Y. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886840

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neuropeptide Y , 0710005A05Rik

Cellular Localization

Secreted

Background

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

Database

Research Area

DATA IMAGES

Anti-Neuropeptide Y antibody used in IHC (Frozen sections) (IHC-Fr). GTX133138

GTX133138 IHC-Fr Image

Neuropeptide Y antibody detects Neuropeptide Y protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Neuropeptide Y stained by Neuropeptide Y antibody (GTX133138) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Neuropeptide Y antibody used in Western Blot (WB). GTX133138

GTX133138 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Neuropeptide Y antibody (GTX133138) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Neuropeptide Y antibody used in IHC (Paraffin sections) (IHC-P). GTX133138

GTX133138 IHC-P Image

Neuropeptide Y antibody detects Neuropeptide Y protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Neuropeptide Y antibody (GTX133138) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Neuropeptide Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133138

GTX133138 ICC/IF Image

Neuropeptide Y antibody detects Neuropeptide Y protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neuropeptide Y stained by Neuropeptide Y antibody (GTX133138) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Grandi V et al. Photodiagnosis Photodyn Ther 2021; 34 : 102222 Single ALA-PDT irradiation induces increase in mast cells degranulation and neuropeptide acute response in chronic venous ulcers: A pilot study.
Application : IHC-P
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169