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Neuropilin 1 antibody

Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947
Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947
Anti-Neuropilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127947
Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947
Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947

Cat No. GTX127947

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

Rat brain , mouse brain , mouse heart , rat heart

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of rat Neuropilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neuropilin 1 , Nrp

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

transmembrane protein that acts as a receptor for semaphorin III; may play a role in neuronal development [RGD]

Database

Research Area

DATA IMAGES

Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947

GTX127947 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuropilin 1 antibody (GTX127947) diluted at 1:1000.

Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947

GTX127947 WB Image

Neuropilin 1 antibody detects Nrp1 protein by Western blot analysis.
A. 50 μg Rat brain lysate/extract
5 % SDS-PAGE
Neuropilin 1 antibody (GTX127947) dilution: 1:1000

Anti-Neuropilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127947

GTX127947 IHC-P Image

Neuropilin 1 antibody detects Neuropilin 1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: Neuropilin 1 antibody (GTX127947) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947

GTX127947 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuropilin 1 antibody (GTX127947) diluted at 1:1000.

Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947

GTX127947 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuropilin 1 antibody (GTX127947) diluted at 1:500.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf