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Neuropilin 1 antibody

Anti-Neuropilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127947
Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947
Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947
Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947
Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947
Anti-Neuropilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127947

Cat. No. GTX127947

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Neuropilin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

Rat brain , mouse brain , mouse heart , rat heart

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of rat Neuropilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885663

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neuropilin 1 , Nrp

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

transmembrane protein that acts as a receptor for semaphorin III; may play a role in neuronal development [RGD]

Database

Research Area

DATA IMAGES

Anti-Neuropilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127947

GTX127947 IHC-P Image

Neuropilin 1 antibody detects Neuropilin 1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: Neuropilin 1 antibody (GTX127947) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947

GTX127947 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuropilin 1 antibody (GTX127947) diluted at 1:1000.

Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947

GTX127947 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuropilin 1 antibody (GTX127947) diluted at 1:500.

Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947

GTX127947 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuropilin 1 antibody (GTX127947) diluted at 1:1000.

Anti-Neuropilin 1 antibody used in Western Blot (WB). GTX127947

GTX127947 WB Image

Neuropilin 1 antibody detects Nrp1 protein by Western blot analysis.
A. 50 μg Rat brain lysate/extract
5 % SDS-PAGE
Neuropilin 1 antibody (GTX127947) dilution: 1:1000

Anti-Neuropilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127947

GTX127947 IHC-P Image

The data was published in the journal Am J Physiol Cell Physiol in 2019. PMID: 30649916

REFERENCE

Application Reference

Mahmoud M et al. Am J Physiol Cell Physiol 2019; (Epub) Smooth muscle cell-specific knock out of Neuropilin-1 impairs postnatal lung development and pathological vascular smooth muscle cell accumulation.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169