APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
IHC-P
Tissues were fixed in paraformaldehyde (PFA) 3.7%, embedded in paraffin and sectioned at 6 mm. The sections were deparaffinized in toluene and hydrated in graded series of ethanol.
FACS
Cells were fixed with ADG fixation buffer A for 15 min at room temperature. Cells were washed and incubated with 10 μg of monoclonal anti-HNE antibody diluted in ADG permeabilisation buffer B.
ELISA
The antibody can be used as capture antibody. Antibody 265-3K1 was unique in that the limit of detection as a capture antibody increased from 625 nM (18.8 ng/ml) for free HNE to 80 nM for HNE–AAT complex, indicating that covalent complex formation between HNE and AAT increases antibody affinity or increases exposure of recognised epitope in HNE for 265-3K1.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ELA2,ELANE,GE,HLE,HNE,NE,PMNE,SCN1,elastase, neutrophil expressed,Neutrophil Elastase
Background
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]
Database
Research Area
REFERENCE
There are currently no references for Neutrophil elastase antibody [265-3K1] (GTX54464). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Neutrophil elastase antibody [265-3K1] (GTX54464). Be the first to share your experience with this product.
