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Neutrophil elastase antibody [AOCI-5]

Anti-Neutrophil elastase antibody [AOCI-5] used in Western Blot (WB). GTX05032
Anti-Neutrophil elastase antibody [AOCI-5] used in Western Blot (WB). GTX05032

Cat. No. GTX05032

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 AOCI-5

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
250 μg ($599)
See all Neutrophil elastase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:100-1:500
FACS 1:30
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 0.04%-0.05% BSA, 50%Glycerol

Preservative

0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Neutrophil Elastase

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ELA2,ELANE,GE,HLE,HNE,NE,PMNE,SCN1,elastase, neutrophil expressed,Neutrophil Elastase

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Neutrophil elastase antibody [AOCI-5] used in Western Blot (WB). GTX05032

GTX05032 WB Image

WB analysis of mouse spleen tissue lysate using GTX05032 Neutrophil elastase antibody [AOCI-5].
Dilution : 1:5000

Anti-Neutrophil elastase antibody [AOCI-5] used in Western Blot (WB). GTX05032

GTX05032 WB Image

WB analysis of various samples using GTX05032 Neutrophil elastase antibody [AOCI-5].
Dilution : 1:500
Loading : 30μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 599