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Neutrophil elastase antibody [N2C3]

Orthogonal
Anti-Neutrophil elastase antibody [N2C3] used in Western Blot (WB). GTX113175
Anti-Neutrophil elastase antibody [N2C3] used in Immunoprecipitation (IP). GTX113175
Anti-Neutrophil elastase antibody [N2C3] used in Western Blot (WB). GTX113175

Cat. No. GTX113175

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Neutrophil elastase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

A549 , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Neutrophil elastase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175419

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

elastase, neutrophil expressed , ELA2 , GE , HLE , HNE , NE , PMN-E , SCN1

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Elastase 2 hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. Elastase 2 may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Neutrophil elastase antibody [N2C3] used in Western Blot (WB). GTX113175

GTX113175 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HepG2
12% SDS PAGE
GTX113175 diluted at 1:1000

Anti-Neutrophil elastase antibody [N2C3] used in Immunoprecipitation (IP). GTX113175

GTX113175 IP Image

Immunoprecipitation of Neutrophil elastase protein from HepG2 whole cell extracts using 5 μg of Neutrophil elastase antibody [N2C3] Neutrop (GTX113175).
Western blot analysis was performed using Neutrophil elastase antibody [N2C3] Neutrop (GTX113175).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Neutrophil elastase antibody [N2C3] used in Western Blot (WB). GTX113175

GTX113175 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Neutrophil elastase antibody [N2C3] (GTX113175) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Martins VF et al. PLoS One 2021; 16 (1) : e0245594 Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169