GeneTex

  • Country / Location Selection

United States (US)

Neutrophil elastase antibody [NP57]

Cat. No. GTX72042

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 NP57

Isotype

 IgG1

Application

 ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human
Package
500 μl ($399)
See all Neutrophil elastase products

APPLICATION

Application Note

IHC: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:150 with AutoProbe III (GTX28620). Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies. The optimal conditions should be determined by the individual laboratory.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Neutrophil Granule Proteins

Purification

Protein A affinity purified

Conjugation

Unconjugated

RRID

AB_383332

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

elastase, neutrophil expressed , ELA2 , GE , HLE , HNE , NE , PMN-E , SCN1

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

Database

Research Area

REFERENCE

Application Reference

Gutor SS et al. Am J Respir Crit Care Med 2022; 206 (3) : 260-270 Characterization of Immunopathology and Small Airway Remodeling in Constrictive Bronchiolitis.
Tsourouktsoglou TD et al. Cell Rep 2020; 31 (5) : 107602 Histones, DNA, and Citrullination Promote Neutrophil Extracellular Trap Inflammation by Regulating the Localization and Activation of TLR4.
Application : ICC/IF
Reactivity : Human
Yang CF et al. Am J Hum Genet 2018; 102 (2) : 219-232 Loss of GPNMB Causes Autosomal-Recessive Amyloidosis Cutis Dyschromica in Humans.
Application : IHC-P
Reactivity : Human
Konnecke M et al. J Immunol Res 2014; 2014 : 959854 Immune imbalance in nasal polyps of Caucasian chronic rhinosinusitis patients is associated with a downregulation of E-selectin.
Hamaguchi S et al. J Inflamm Res 2012; 5 : 137-40 Case of invasive nontypable Haemophilus influenzae respiratory tract infection with a large quantity of neutrophil extracellular traps in sputum.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Neutrophil elastase antibody [NP57] (GTX72042). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399